Amrita Centre for Nanoscience and Molecular Medicine, Amrita Institute of Medical Sciences, Amrita Vishwa Vidyapeetham University, Cochin, Kerala, India.
Nanotoxicology. 2012 Sep;6(6):652-66. doi: 10.3109/17435390.2011.600839. Epub 2011 Jul 22.
Multimodal molecular imaging provides both anatomical and molecular information, aiding early stage detection and better treatment planning of diseased conditions. Here, we report development and nanotoxicity evaluation of a novel hydroxyapatite nanoparticle (nHAp) based multimodal contrast agent for combined near-infrared (NIR), MR and X-ray imaging. Under optimised wet-chemical conditions, we achieved simultaneous doping of nHAp (size ∼50 nm) with indocyanine green and Gd(3+) contributing to NIR contrast (∼750-850 nm), paramagnetic behaviour and X-ray absorption suitable for NIR, MR and X-ray contrast imaging, respectively. Haematocompatibility studies using stem cell viability, haemolysis, platelet activation, platelet aggregation and coagulation time analysis indicated excellent compatibility of doped nHAp (D-nHAp). Further, the immunogenic function studies using human lymphocytes (in vitro) showed that D-nHAp caused no adverse effects. Collectively, our studies suggest that D-nHAp with excellent biocompatibility and multifunctional properties is a promising nanocontrast agent for combined NIR, MR and X-ray imaging applications.
多模态分子成像提供解剖学和分子信息，有助于早期发现病变，并为病变情况制定更好的治疗计划。在这里，我们报告了一种新型基于羟基磷灰石纳米粒子（nHAp）的多模态对比剂的开发和纳米毒性评估，用于联合近红外（NIR）、磁共振（MR）和 X 射线成像。在优化的湿化学条件下，我们实现了 nHAp（尺寸约为 50nm）与吲哚菁绿和 Gd（3+）的同时掺杂，分别为近红外对比（～750-850nm）、顺磁行为和 X 射线吸收提供贡献，适用于近红外、MR 和 X 射线对比成像。使用干细胞活力、溶血、血小板激活、血小板聚集和凝血时间分析进行的血液相容性研究表明，掺杂的 nHAp（D-nHAp）具有极好的相容性。此外，使用人淋巴细胞（体外）进行的免疫原性功能研究表明，D-nHAp 没有不良影响。总的来说，我们的研究表明，具有优异的生物相容性和多功能特性的 D-nHAp 是一种很有前途的用于联合近红外、磁共振和 X 射线成像应用的纳米对比剂。
