Department of Ophthalmology, Fatih University Medical School, Ankara, Turkey.
Curr Eye Res. 2011 Aug;36(8):713-8. doi: 10.3109/02713683.2011.584370.
The aim of this study was to establish the antioxidant status and oxidative stress in patients with primary open-angle glaucoma (POAG) and pseudoexfoliative glaucoma (PEG).
Serum levels of total antioxidant capacity (TAC) and superoxide dismutase (SOD) as indicators of antioxidant status; and total oxidant status (TOS), nitric oxide (NO), protein carbonyl (PC), and malondialdehyde (MDA) as indicators of oxidative stress were measured from the blood samples of patients with POAG (n = 23), PEG (n = 24) and healthy control subjects (n = 19) by spectrophotometry.
Mean TAC level was 0.6 ± 0.1 mmol/L in the POAG group; 0.5 ± 0.1 mmol/L in the PEG group and 1.2 ± 0.3 mmol/L in the control group (p = 0.001). Mean SOD level was 13 ± 0.5 mg/L in the POAG group, 11.6 ± 0.2 mg/L in the PEG group and 9.4 ± 0.6 mg/L in the control group (p = 0.001). Mean TOS level was 19.6 ± 2.6 μmol/L in the POAG group, 21.2 ± 4.2 μmol/L in the PEG group and 15.1 ± 7 μmol/L in the control group (p = 0.001). Mean NO level was 74.3 ± 14.4 µmol/L in the POAG group, 66.1 ± 8.1 µmol/L in the PEG group and 62.3 ± 13.5 µmol/L in the control group (p = 0.005). Mean PC level was 641.5 ± 102.5 nmol/mg in the POAG group, 988.3 ± 214.7 nmol/mg in the PEG group and 654.4 ± 150.7 nmol/mg in the control group (p = 0.001). Mean MDA level was 1.9 ± 0.2 µmol/L in the POAG group, 1.7 ± 0.4 µmol/L in the PEG group and 1.1 ± 0.2 µmol/L in the control group (p = 0.001).
The findings of the present study are potentially of significance and add to the growing body of evidence for oxidative stress in POAG and PEG. Decreased antioxidant defense and increased oxidative stress system may play an important role in the pathogenesis of POAG and PEG.
本研究旨在确定原发性开角型青光眼（POAG）和假性剥脱性青光眼（PEG）患者的抗氧化状态和氧化应激情况。
通过分光光度法检测 POAG 患者（n=23）、PEG 患者（n=24）和健康对照组（n=19）的血清总抗氧化能力（TAC）和超氧化物歧化酶（SOD）水平，以评估抗氧化状态；检测总氧化状态（TOS）、一氧化氮（NO）、蛋白羰基（PC）和丙二醛（MDA）水平，以评估氧化应激情况。
POAG 组的平均 TAC 水平为 0.6±0.1mmol/L；PEG 组为 0.5±0.1mmol/L；对照组为 1.2±0.3mmol/L（p=0.001）。POAG 组的平均 SOD 水平为 13±0.5mg/L；PEG 组为 11.6±0.2mg/L；对照组为 9.4±0.6mg/L（p=0.001）。POAG 组的平均 TOS 水平为 19.6±2.6μmol/L；PEG 组为 21.2±4.2μmol/L；对照组为 15.1±7μmol/L（p=0.001）。POAG 组的平均 NO 水平为 74.3±14.4μmol/L；PEG 组为 66.1±8.1μmol/L；对照组为 62.3±13.5μmol/L（p=0.005）。POAG 组的平均 PC 水平为 641.5±102.5nmol/mg；PEG 组为 988.3±214.7nmol/mg；对照组为 654.4±150.7nmol/mg（p=0.001）。POAG 组的平均 MDA 水平为 1.9±0.2μmol/L；PEG 组为 1.7±0.4μmol/L；对照组为 1.1±0.2μmol/L（p=0.001）。
本研究的结果具有重要意义，为 POAG 和 PEG 中的氧化应激提供了更多证据。抗氧化防御能力下降和氧化应激系统增强可能在 POAG 和 PEG 的发病机制中发挥重要作用。
