假性剥脱性青光眼和原发性开角型青光眼硫醇-二硫键稳态的评估。
Evaluation of thiol-disulfide homeostasis in pseudoexfoliation glaucoma and primary open-angle glaucoma.
作者信息
Cetinkaya E, Duman R, Sabaner M C, Erol M A, Duman R, Nural C, Erel O
机构信息
Ophthalmology, Antalya Training and Research Hospital, Antalya, Turkey.
Department of Ophthalmology, Kocatepe University Faculty of Medicine, Afyonkarahisar, Turkey.
出版信息
Niger J Clin Pract. 2020 Oct;23(10):1401-1406. doi: 10.4103/njcp.njcp_452_19.
AIMS
This study aimed to compare the serum thiol-disulfide homeostasis, total antioxidant status (TAS), and total oxidant status (TOS) in patients with pseudoexfoliation glaucoma (PEG) patients, primary open-angle glaucoma (POAG) patients, and healthy individuals (control).
METHODS
Ninety subjects were included in this study. Three groups were separated as PEG, POAG, and control. All groups were chosen to be similar in terms of age and gender. Blood samples were obtained following an overnight fasting state and were collected on the ice at 4°C. The serum samples were separated from the cells by centrifugation at 3000 rpm for 15 min and were stored at -80°C. Serum samples analyzed for TAS and TOS, native thiol, total thiol, disulfide, and native thiol/disulfide ratio.
RESULTS
TAS and TOS levels of PEG patients were 1.2892 ± 0.0905 mmol/L; 5.0191 ± 2.7722 μmol/L, respectively. TAS and TOS levels of POAG patients were 1.2741 ± 0.1252 mmol/L; 4.1674 ± 1.7723 μmol/L, respectively. TAS and TOS levels of the control group were 2.3414 ± 0.1409 mmol/L; 4.0931 ± 0.1107 μmol/L, respectively. The TAS level was significantly lower in PEG and POAG groups compared to control. TOS level showed no significant differ ¬ ence between PEG, POAG, and control groups (P > 0.05). The mean serum total thiol and native thiol levels were significantly lower in patients with PEG compared to POAG and control group; there was no significant difference between the POAG and control group (P > 0.05). The mean serum disulfide level was significantly lower in patients with PEG compared to POAG (P = 0.018).
CONCLUSION
Low levels of TAS were observed in patients with glaucoma, which was likely a response to the increased oxidative stress observed in these patients. While total thiol and native thiol levels were higher in the PEG group, the disulfide level was higher in the POAG group. TAS and TOS levels showed no significant difference between POAG and PEG groups.
目的
本研究旨在比较剥脱性青光眼（PEG）患者、原发性开角型青光眼（POAG）患者和健康个体（对照组）的血清硫醇 - 二硫键稳态、总抗氧化状态（TAS）和总氧化状态（TOS）。
方法
本研究纳入90名受试者。分为PEG组、POAG组和对照组。所有组在年龄和性别方面选择相似。在过夜禁食状态后采集血样，并在4°C的冰上收集。通过以3000 rpm离心15分钟将血清样品与细胞分离，并储存在-80°C。分析血清样品中的TAS、TOS、天然硫醇、总硫醇、二硫键和天然硫醇/二硫键比率。
结果
PEG患者的TAS和TOS水平分别为1.2892±0.0905 mmol/L；5.019 I±2.7722 μmol/L。POAG患者的TAS和TOS水平分别为1.2741±0.1252 mmol/L；4.1674±1.7723 μmol/L。对照组的TAS和TOS水平分别为2.3414±0.1409 mmol/L；4.0931±0.1107 μmol/L。与对照组相比，PEG组和POAG组的TAS水平显著降低。PEG组、POAG组和对照组之间的TOS水平无显著差异（P>0.05）。与POAG组和对照组相比，PEG患者的平均血清总硫醇和天然硫醇水平显著降低；POAG组和对照组之间无显著差异（P>0.05）。与POAG组相比，PEG患者的平均血清二硫键水平显著降低（P = 0.018）。
结论
青光眼患者中观察到TAS水平较低，这可能是对这些患者中观察到的氧化应激增加的一种反应。虽然PEG组的总硫醇和天然硫醇水平较高，但POAG组的二硫键水平较高。POAG组和PEG组之间的TAS和TOS水平无显著差异。
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