[浙江省龙泉市小型哺乳动物中汉坦病毒自然感染状况调查]
[Investigation on the natural infectious status of hantaviruses among small mammals in Longquan city, Zhejiang province].
作者信息
Wang Miao-ruo, Wang Wen, Lin Xian-dan, Mei Sheng-hua, Guo Wen-ping, Zhang Yong-zhen
机构信息
Zhejiang Provincial of Longquan Center for Disease Control and Prevention, Longquan, China.
出版信息
Zhonghua Liu Xing Bing Xue Za Zhi. 2011 Jun;32(6):598-601.
OBJECTIVE
To investigate the situation of the natural infection of hantaviruses (HV) in small mammals and to provide evidence for the control and prevention of hemorrhagic fever with renal syndrome (HFRS) in Longquan area, Zhejiang province.
METHODS
Small mammals were captured by night trap, and lung tissue samples were collected and stored in liquid nitrogen. HV antigens were detected by indirect immuno-fluorescence assay (IFA). The partial S genome segment sequences were amplified by RT-PCR. DNAStar program was used for editing and comparing the sequences. Phylogeny was analyzed through PAUP*4.0 software.
RESULTS
319 small animals were collected in Longquan, and 9 hantavirus antigen-positive samples were identified. The positive rate of hantavirus in Apodemus agrarius was 4.97%. Phylogenetic tree constructed by partial S segment (620 - 999 nt) showed that the 9 strains carried by A. agrarius from Longquan all belonged to HTNV, and had a closer evolutionary relationship with isolate Z251 from Zhejiang province.
CONCLUSION
Our results indicated that the main host was A. agrarius and the infection rate of HTNV was high in Longquan area.
目的
调查小型哺乳动物中汉坦病毒（HV）的自然感染情况，为浙江省龙泉地区肾综合征出血热（HFRS）的防控提供依据。
方法
采用夜捕法捕获小型哺乳动物，采集肺组织样本并保存于液氮中。采用间接免疫荧光法（IFA）检测HV抗原。通过RT-PCR扩增部分S基因组片段序列。使用DNAStar程序对序列进行编辑和比较。通过PAUP*4.0软件进行系统发育分析。
结果
在龙泉共捕获319只小型动物，鉴定出9份汉坦病毒抗原阳性样本。黑线姬鼠中汉坦病毒阳性率为4.97%。由部分S片段（620 - 999 nt）构建的系统发育树显示，龙泉地区黑线姬鼠携带的9株病毒均属于汉滩病毒（HTNV），且与浙江省分离株Z251进化关系较近。
结论
结果表明，龙泉地区主要宿主为黑线姬鼠，HTNV感染率较高。
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