Delaware Biotechnology Institute Bio-Imaging Center, University of Delaware, Newark, DE 19711, United States.
Curr Opin Struct Biol. 2011 Oct;21(5):686-93. doi: 10.1016/j.sbi.2011.06.010. Epub 2011 Jul 21.
Correlative microscopy is a sophisticated approach that combines the capabilities of typically separate, but powerful microscopy platforms: often including, but not limited, to conventional light, confocal and super-resolution microscopy, atomic force microscopy, transmission and scanning electron microscopy, magnetic resonance imaging and micro/nano CT (computed tomography). When targeting rare or specific events within large populations or tissues, correlative microscopy is increasingly being recognized as the method of choice. Furthermore, this multi-modal assimilation of technologies provides complementary and often unique information, such as internal and external spatial, structural, biochemical and biophysical details from the same targeted sample. The development of a continuous stream of cutting-edge applications, probes, preparation methodologies, hardware and software developments will enable realization of the full potential of correlative microscopy.
相关显微镜技术是一种复杂的方法，它结合了通常独立但功能强大的显微镜平台的能力：通常包括但不限于传统的光、共聚焦和超分辨率显微镜、原子力显微镜、透射和扫描电子显微镜、磁共振成像和微/纳米 CT（计算机断层扫描）。当针对大群体或组织中的罕见或特定事件时，相关显微镜技术越来越被认为是首选方法。此外，这种多模式技术的融合提供了互补且通常独特的信息，例如来自同一靶向样本的内部和外部空间、结构、生化和生物物理细节。不断涌现的前沿应用、探针、制备方法学、硬件和软件的发展，将使相关显微镜技术的全部潜力得以实现。
