College of Pharmacy, Sookmyung Women's University, 53-12 Chungpa-dong 2 Ka, Yongsan-ku, Seoul 140-742, Korea.
Environ Toxicol Pharmacol. 2005 Jan;19(1):19-24. doi: 10.1016/j.etap.2004.02.006.
Bisphenol A (BPA) is known to have detrimental effects on the reproductive system, but the toxicity of BPA on immune responses has not been systematically investigated. We investigated the effects of BPA exposure on the activities of murine peritoneal macrophages through evaluation of BPA-induced alteration of nitric oxide (NO) production, tumor necrosis factor-α (TNF-α) synthesis, and expression of co-stimulatory molecules B7. Macrophages were examined ex vivo from mice orally treated with various doses of BPA for 5 consecutive days per week for 4 weeks followed by culture for 2 or 4 days in the presence of lipopolysaccharides (LPS). Macrophages from naive mice were also stimulated with LPS ± BPA for 2 or 4 days. NO production was decreased with the in vitro exposure to 1, 10 and 100μM BPA. NO production was lower in the BPA-exposed mice than the control mice with all doses. In vitro, BPA suppressed TNF-α secretion with significant reduction at 10 and 100μM BPA. Similar findings were observed with the macrophages from the BPA-exposed mice. This study provides the substantial evidence on BPA-induced alteration in macrophage activity.
双酚 A（BPA）已知对生殖系统有有害影响，但 BPA 对免疫反应的毒性尚未得到系统研究。我们通过评估 BPA 诱导的一氧化氮（NO）产生、肿瘤坏死因子-α（TNF-α）合成和共刺激分子 B7 的表达变化，研究了 BPA 暴露对小鼠腹腔巨噬细胞活性的影响。用不同剂量的 BPA 连续 5 天每天口服处理小鼠，持续 4 周，然后在脂多糖（LPS）存在下培养 2 或 4 天，对巨噬细胞进行离体检查。用 LPS±BPA 对来自未处理的小鼠的巨噬细胞进行刺激 2 或 4 天。体外接触 1、10 和 100μM BPA 可降低 NO 的产生。与对照组相比，所有剂量的 BPA 暴露小鼠的 NO 产生均较低。体外，BPA 抑制 TNF-α 的分泌，在 10 和 100μM BPA 时显著减少。在来自 BPA 暴露小鼠的巨噬细胞中也观察到了类似的发现。这项研究提供了 BPA 诱导的巨噬细胞活性改变的实质性证据。
