San Jose Creek Water Quality Control Laboratory, Sanitation Districts of Los Angeles County, 1965 Workman Mill Road, Whittier, CA 90601, USA.
Environ Toxicol Pharmacol. 2005 Sep;20(2):269-78. doi: 10.1016/j.etap.2005.01.008. Epub 2005 Mar 23.
Ozonation and chlorination of 17β-estradiol (E2), 17α-ethynylestradiol (EE2), bisphenol A (BPA), and nonylphenol (NP) were performed to evaluate the estrogenic activity of the by-products of these endocrine disrupting chemicals (EDCs). After 15min oxidation, samples were extracted using solid phase extraction (SPE) cartridges, and tested in vitro to measure the estrogenic activities of the oxidized products. MCF-7 cell proliferation assay showed that chlorinated BPA solution displayed slightly stronger estrogenicity than BPA, while chlorinated NP retained about one-tenth of its bioactivity. The estrogenic mono-, di-, tri-, and tetra-ClBPAs and di-ClNP were screened out from the corresponding chlorinated products by a combined application of estrogen receptor (ER) binding with ultrafiltration and identified by high performance liquid chromatography coupled with mass spectrometry (LC/MS). Ozonation of the above four estrogens and chlorination of E2 and EE2 significantly decreased their estrogenic activities under the applied conditions.
对 17β-雌二醇（E2）、17α-乙炔基雌二醇（EE2）、双酚 A（BPA）和壬基酚（NP）进行了臭氧化和氯化处理，以评估这些内分泌干扰化学物质（EDCs）的副产物的雌激素活性。氧化 15 分钟后，使用固相萃取（SPE）小柱提取样品，并进行体外测试以测量氧化产物的雌激素活性。MCF-7 细胞增殖测定表明，氯化 BPA 溶液显示出比 BPA 略强的雌激素活性，而氯化 NP 保留了约十分之一的生物活性。通过将雌激素受体（ER）结合超滤和高效液相色谱-质谱联用（LC/MS）相结合，从相应的氯化产物中筛选出雌激素单氯、二氯、三氯和四氯 BPA 以及二氯 NP。在应用条件下，上述四种雌激素的臭氧化和 E2 和 EE2 的氯化显著降低了它们的雌激素活性。
