# 521, Molecular Biochemistry Laboratory, Institute of Biotechnology, Chonnam National University, 300 Yongbong-Dong, Kwangju 500-757, South Korea.
Environ Toxicol Pharmacol. 2006 Jul;22(1):8-14. doi: 10.1016/j.etap.2005.10.005. Epub 2006 Jan 11.
This study was carried out to investigate the hepatoprotective effect of the glycoprotein isolated from Rhus verniciflua Stokes (RVS), which has traditionally been used for healing of inflammatory diseases. We evaluated the activities of alanine aminotransferase (ALT), lactate dehydrogenase (LDH), thiobarbituric acid-reactive substances (TBARS), and antioxidant enzymes [superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx)] activities in treatment with carbon tetrachloride (CCl(4)) in vivo. When mice were treated with CCl(4) in the absence of RVS glycoprotein, the activities of ALT, LDH, and TBARS were increased, while the antioxidant enzymes activities were decreased. However, when the mice were treated with CCl(4) in the presence of RVS glycoprotein, the activities of ALT, LDH, and TBARS were significantly reduced and SOD, CAT, and GPx activities were remarkably increased. In addition, RVS glycoprotein increased the nitric oxide (NO) production and decreased the nuclear factor-kappa B (NF-κB) and activator protein-1 (AP-1) activation in CCl(4)-treated mice. Collectively, these results pointed out that RVS glycoprotein can inhibit lipid peroxidation, enhance the activities of antioxidant enzymes, increase the NO production, and decrease the NF-κB and AP-1 activations. Therefore, we speculate that RVS glycoprotein protects from liver damage through its radical scavenging ability.
本研究旨在探讨漆树糖蛋白（RVS）的保肝作用，漆树糖蛋白传统上用于治疗炎症性疾病。我们评估了丙氨酸转氨酶（ALT）、乳酸脱氢酶（LDH）、硫代巴比妥酸反应物（TBARS）和抗氧化酶[超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPx）]在体内四氯化碳（CCl(4)）处理中的活性。当小鼠在缺乏 RVS 糖蛋白的情况下用 CCl(4)处理时，ALT、LDH 和 TBARS 的活性增加，而抗氧化酶的活性降低。然而，当小鼠在存在 RVS 糖蛋白的情况下用 CCl(4)处理时，ALT、LDH 和 TBARS 的活性显著降低，SOD、CAT 和 GPx 的活性显著增加。此外，RVS 糖蛋白增加了 CCl(4)处理小鼠的一氧化氮（NO）产生，并降低了核因子-κB（NF-κB）和激活蛋白-1（AP-1）的激活。总之，这些结果表明 RVS 糖蛋白可以通过其清除自由基的能力抑制脂质过氧化，增强抗氧化酶的活性，增加 NO 的产生，并降低 NF-κB 和 AP-1 的激活。因此，我们推测 RVS 糖蛋白通过其清除自由基的能力来保护肝脏免受损伤。
