Equipe des Sciences Séparatives et Biopharmaceutiques (EA 3924), Université de Franche-Comté UFR des Sciences Médicales et Pharmaceutiques, Laboratoire de Biologie Cellulaire, Place Saint Jacques, F-25030 Besançon Cedex, France.
Environ Toxicol Pharmacol. 2006 Jul;22(1):27-34. doi: 10.1016/j.etap.2005.11.003. Epub 2006 Jan 20.
Roundup 3 plus(®), a glyphosate-based herbicide, is widely used in the ground, but its extensive use has posed a health risk in man. The aim of this study was firstly to investigate how glyphosate alone or included in Roundup 3 plus(®) affected the antioxidant defense system and lipid peroxidation of human cutaneous cells, and secondly, to evaluate the ameliorating effects of antioxidants, as Vitamin C (VitC) and Vitamin E (VitE), against Roundup 3 plus(®)-induced epidermal antioxidant impairment. Our results showed that glyphosate alone or included in Roundup 3 plus(®), induced significant changes in cellular antioxidant status as a glutathione depletion, enzymatic (catalase, glutathione-peroxidase and superoxide dismutase) disorders, and increased lipid peroxidation. VitC or VitE supplementation increased superoxide dismutase, glutathione-reductase and -peroxidase activities and reduced lipid peroxidation in Roundup 3 plus(®)-treated keratinocytes. These in vitro data indicated that VitC and VitE might have preventive effects against deleterious cutaneous cell damage caused by Roundup 3 plus(®).
农达 3 号 Plus（®）是一种广泛应用于地面的草甘膦除草剂，但它的大量使用对人类健康构成了威胁。本研究的目的首先是调查草甘膦单独或包含在农达 3 号 Plus（®）中如何影响人类皮肤细胞的抗氧化防御系统和脂质过氧化，其次，评估抗氧化剂（如维生素 C（VitC）和维生素 E（VitE））对农达 3 号 Plus（®）诱导的表皮抗氧化损伤的改善作用。我们的结果表明，草甘膦单独或包含在农达 3 号 Plus（®）中，会导致细胞抗氧化状态发生显著变化，如谷胱甘肽耗竭、酶（过氧化氢酶、谷胱甘肽过氧化物酶和超氧化物歧化酶）紊乱以及脂质过氧化增加。VitC 或 VitE 补充可增加超氧化物歧化酶、谷胱甘肽还原酶和过氧化物酶的活性，并减少农达 3 号 Plus（®）处理的角质形成细胞中的脂质过氧化。这些体外数据表明，VitC 和 VitE 可能对农达 3 号 Plus（®）引起的有害皮肤细胞损伤具有预防作用。
