Department of Obstetrics and Gynecology, Faculty of Medicine, Süleyman Demirel University, Isparta, Turkey.
Environ Toxicol Pharmacol. 2007 Mar;23(2):221-7. doi: 10.1016/j.etap.2006.10.002. Epub 2006 Oct 17.
The role of reactive oxygen species (ROS) in various diseases of the female reproductive tract has been shown, and oxidative stress is an important component of the mechanism of toxicity of OPIs. Methyl parathion (MPT) is one of the most widely used organophosphate insecticides (OPIs) in agriculture. The aim of the study was to elucidate the effect of subchronic MPT exposure on lipid peroxidation and serum activities of cholinesterase (ChE), and the protective effects of combination of antioxidant Vitamins E and C in rats. Additionally, histopathological and immunohistochemical changes in endometrium were aimed to be examined. Three groups of rats were used in the experiment. The first group was treated with 5mg/kg MPT; the second group was treated with 5mg/kg body weight MPT plus Vitamin E and Vitamin C (MPT+Vit); and the third group was given only corn oil (control). MPT and MPT+Vit groups were given MPT by gavage 5 days a week for 4 weeks at a dose level of 4mg/(kgday) by using corn oil as the vechicle. Vitamins E and C were injected at doses of 50mg/kg i.m. and 20mg/kg body weight i.p. Histopathological and immunohistochemical examinations for caspase-3 and caspase-9 were accomplished in the endometrium. The level of malondialdehyde (MDA) increased significantly in the MPT group compared with the control group (p<0.05). MDA significantly decreased in the MPT+Vit group compared with the MPT group (p<0.05). Administration of Vitamins E and C along with MPT significantly reduced the histopathological changes and the extent of apoptosis. In conclusion, subchronic MPT administration caused endometrial damage and that treatment with a combination of Vitamins E and C reduced endometrial damage caused by MPT.
活性氧（ROS）在女性生殖道的各种疾病中起作用，氧化应激是有机磷杀虫剂（OPIs）毒性机制的重要组成部分。甲基对硫磷（MPT）是农业中使用最广泛的有机磷杀虫剂之一。本研究旨在阐明亚慢性 MPT 暴露对脂质过氧化和血清胆碱酯酶（ChE）活性的影响，以及抗氧化维生素 E 和 C 联合使用对大鼠的保护作用。此外，还旨在检查子宫内膜的组织病理学和免疫组织化学变化。实验中使用了三组大鼠。第一组用 5mg/kg MPT 处理；第二组用 5mg/kg 体重 MPT 加维生素 E 和维生素 C（MPT+Vit）处理；第三组仅给予玉米油（对照）。MPT 和 MPT+Vit 组用玉米油作为载体，每周 5 天通过灌胃给予 MPT，剂量为 4mg/（kg·天），共 4 周。维生素 E 和 C 以 50mg/kg 肌肉注射和 20mg/kg 体重腹腔注射的剂量给予。在子宫内膜中完成了 caspase-3 和 caspase-9 的组织病理学和免疫组织化学检查。与对照组相比，MPT 组的丙二醛（MDA）水平显著升高（p<0.05）。与 MPT 组相比，MPT+Vit 组的 MDA 显著降低（p<0.05）。与 MPT 一起给予维生素 E 和 C 显著减少了组织病理学变化和细胞凋亡的程度。总之，亚慢性 MPT 给药导致子宫内膜损伤，而维生素 E 和 C 的联合治疗可减少 MPT 引起的子宫内膜损伤。
