绿原酸对甲基汞诱导的 PC12 细胞凋亡的神经保护作用。
Neuroprotective effects of chlorogenic acid against apoptosis of PC12 cells induced by methylmercury.
机构信息
Department of Nutrition and Food Hygiene, Health Science Center, Peking University, Beijing 100083, PR China; School of Public Health, Jilin Medical College, Jilin 132013, PR China.
出版信息
Environ Toxicol Pharmacol. 2008 Jul;26(1):13-21. doi: 10.1016/j.etap.2007.12.008. Epub 2008 Jan 19.
Chlorogenic acid (CGA) widely exists in edible and medicinal plants. We aimed to evaluate the effect of CGA on the protection from apoptosis by methylmercury (MeHg) in PC12 cells. Cell viability was evaluated by MTT assay. Apoptosis was assayed by flow cytometry detection. Caspase-3 activity was measured by confocal microscopy. Intracellular GSH levels were determined by bicinchoninic acid protein assay. Intracellular reactive oxygen species (ROS) was assessed by means of chloromethyl-dihydrodichlorofluorescein diacetate. Glutathione peroxidase (GPx) activity was determined by UV. In order to elucidate the action of CGA, the protective effects of CGA were compared to Vit.E. CGA was effective at protecting PC12 cells against MeHg-induced damage in dose-dependent manner. CGA not only suppressed the generation of ROS, the decrease of activity in GPx and the decrease of GSH, but also attenuated caspase-3 activation in PC12 cells by MeHg. CGA eventually protected PC12 cells against MeHg-induced apoptosis. The results highlighted that CGA may exert neuroprotective effects through its antioxidant actions.
绿原酸（CGA）广泛存在于食用和药用植物中。我们旨在评估 CGA 对甲基汞（MeHg）诱导的 PC12 细胞凋亡的保护作用。通过 MTT 法评估细胞活力。通过流式细胞术检测凋亡。通过共聚焦显微镜测量 caspase-3 活性。通过双缩脲蛋白测定法测定细胞内 GSH 水平。通过氯甲基二氢二氯荧光素二乙酸酯评估细胞内活性氧（ROS）。通过 UV 测定谷胱甘肽过氧化物酶（GPx）活性。为了阐明 CGA 的作用，将 CGA 的保护作用与 Vit.E 进行了比较。CGA 能够以剂量依赖性方式有效保护 PC12 细胞免受 MeHg 诱导的损伤。CGA 不仅抑制了 ROS 的产生、GPx 活性的降低和 GSH 的减少，而且还减弱了 MeHg 诱导的 PC12 细胞中 caspase-3 的激活。CGA 最终保护 PC12 细胞免受 MeHg 诱导的凋亡。结果表明，CGA 可能通过其抗氧化作用发挥神经保护作用。
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