Institute of Biomedical Nutrition, Hung Kuang University, Taichung 433, Taiwan, ROC.
Environ Toxicol Pharmacol. 2009 Mar;27(2):176-81. doi: 10.1016/j.etap.2008.10.001. Epub 2008 Oct 15.
Present work was carried out to investigate how testicular selenium (Se) metabolisms respond to oxidative stress induced by aluminum (Al). Mice were intraperitoneally exposed to 0, 7, or 35mg Al/kg/d for 14 days (CNL, LAL and HAL groups). Al administration significantly increased Al, reactive oxygen radical and malondialdehyde (MDA) levels, as well as decreased glutathione peroxidase (GPx) and glutathione reductase (GR) activities in serum and testes. The serum concentrations of Se were remarkably lower at LAL and HAL groups compared to the controls, whereas the testicular Se levels significantly reduced only in the HAL group. In addition, RT-PCR analysis revealed an increased testicular selenoprotein P (SelP) expression by Al treatment. Western blot analysis showed increased levels of SelP protein expression in the LAL group, but the expression levels were significantly reduced in HAL group. It was suggested that altered metabolism of Se, further stimulated testicular SelP transcription that may compensate for the loss of SelP protein resulted from Al-induced oxidative damage.
本研究旨在探讨睾丸硒（Se）代谢如何应对铝（Al）诱导的氧化应激。将小鼠经腹腔注射分别给予 0、7 或 35mg Al/kg/d，连续 14 天（CNL、LAL 和 HAL 组）。Al 处理显著增加了血清和睾丸中 Al、活性氧自由基和丙二醛（MDA）的水平，同时降低了血清和睾丸中谷胱甘肽过氧化物酶（GPx）和谷胱甘肽还原酶（GR）的活性。与对照组相比，LAL 和 HAL 组的血清 Se 浓度显著降低，而仅在 HAL 组中睾丸 Se 水平显著降低。此外，RT-PCR 分析显示 Al 处理增加了睾丸硒蛋白 P（SelP）的表达。Western blot 分析显示，LAL 组 SelP 蛋白表达水平升高，但在 HAL 组表达水平显著降低。这表明，Se 代谢的改变进一步刺激了睾丸 SelP 转录，可能补偿了 Al 诱导的氧化损伤导致的 SelP 蛋白丢失。
