BK21 Oriental Medical Science Center, KyungHee University, Seoul 130-701, Republic of Korea.
Environ Toxicol Pharmacol. 2009 Mar;27(2):225-30. doi: 10.1016/j.etap.2008.10.009. Epub 2008 Oct 28.
This study was conducted to quantitatively evaluate the recovery effects of herbal medicines on acetaminophen-induced nephrotoxicity. In the present study, the recovery effects of 251 herb medicines on HEK 293 cells that had been damaged by acetaminophen were evaluated using an MTS assay. HEK 293 cells were cultured in 96-well plates and then pretreated with or without 20μM acetaminophen (IC(50) value: 17.5±1.9) for 1h. Next, different herbal medicines were added to the wells, after which the cells were reincubated at 37°C for 24h. After the first round of screening, the candidate herbal medicines were selected based on a recovery rate of greater than 20% and their efficacy were then determined by dose response kinetic analysis. Among these extracts, 8 herbal medicines (Ledebouriella divaricata, Sparganium simplex, Panax ginseng, Aster tataricus, Citrus aurantium, Sanguisorba officianlis, Arisaema consanguineum, and Polygonum aviculare) had a strong recovery effect on acetaminophen-induced damage in HEK 293 cells. Dose response non-linear regression analysis demonstrated that P. aviculare showed the best recovery rate (98%), and that its EC(50) (0.1ng/mL) was the smallest among the screened candidate herbal medicines. Additional studies of these herbal medicines should be conducted to determine if they possess novel therapeutic agents for the prevention or treatment of renal disorders.
本研究旨在定量评估草药对乙酰氨基酚诱导的肾毒性的恢复作用。在本研究中，使用 MTS 测定法评估了 251 种草药对已被乙酰氨基酚损伤的 HEK 293 细胞的恢复作用。HEK 293 细胞在 96 孔板中培养，然后用或不用 20μM 乙酰氨基酚（IC 50 值：17.5±1.9）预处理 1 小时。然后将不同的草药添加到孔中，然后在 37°C 下再孵育 24 小时。在第一轮筛选后，根据大于 20%的恢复率选择候选草药，并通过剂量反应动力学分析确定其疗效。在这些提取物中，有 8 种草药（鹿蹄草、水葱、人参、蒲公英、枳实、地榆、藜芦、虎杖）对 HEK 293 细胞中乙酰氨基酚诱导的损伤具有很强的恢复作用。剂量反应非线性回归分析表明，虎杖的恢复率最高（98%），其 EC 50 （0.1ng/mL）在筛选出的候选草药中最小。应对这些草药进行进一步研究，以确定它们是否具有预防或治疗肾脏疾病的新型治疗剂。
