Department of Zoology, Karnatak University, Dharwad-580 003, India.
Environ Toxicol Pharmacol. 2009 May;27(3):390-5. doi: 10.1016/j.etap.2009.01.002. Epub 2009 Jan 29.
Effects of ibuprofen (a non-selective COX inhibitor) on the embryonic development, hatching success, larval growth, behavioral pattern and survival competence were studied in Danio rerio. Embryos at 2/4 celled stage were exposed to graded doses (0, 1, 5, 10, 50 and 100μg/L distilled water) of ibuprofen in triplicate sets (n=30). The experiment was repeated thrice. The results indicate that developing embryos tolerated lower (1 and 5μg/L) doses of the drug readily but, exposure to higher doses (>10μg/L) caused retarded development, decreased hatching rate and growth, cardiac anomalies, spinal curvature, pectoral fin malformation and behavioral alterations resulting in greater mortality of experimental embryos. This study suggests that, ibuprofen which is marketed as over-the-counter (OTC) drug is embryotoxic at least at higher (>10μg/L) dose level to zebrafish embryos.
在斑马鱼胚胎中研究了布洛芬（一种非选择性 COX 抑制剂）对胚胎发育、孵化成功率、幼虫生长、行为模式和生存能力的影响。将处于 2/4 细胞期的胚胎暴露于不同浓度（0、1、5、10、50 和 100μg/L 蒸馏水）的布洛芬中，每组重复三次（n=30）。结果表明，发育中的胚胎能够轻易耐受较低（1 和 5μg/L）剂量的药物，但暴露于较高剂量（>10μg/L）会导致发育迟缓、孵化率和生长下降、心脏异常、脊柱弯曲、胸鳍畸形和行为改变，从而导致实验胚胎的死亡率增加。本研究表明，布洛芬作为非处方（OTC）药物，至少在较高（>10μg/L）剂量水平对斑马鱼胚胎具有胚胎毒性。
