Atatürk University, Science and Art Faculty, Biology Department, 25240 Erzurum, Turkey.
Environ Toxicol Pharmacol. 2008 Jan;25(1):83-8. doi: 10.1016/j.etap.2007.09.001. Epub 2007 Sep 17.
The present study examines the effects of PCN congeners (PCN50 and PCN66) on zebrafish embryos. The embryos were exposed to different concentrations (20, 30 and 50μg/l) of the respective congeners by dissolving 0.08% acetone. The following endpoints were investigated (mortality, hatching rate, abnormalities and heart rate). No effect on survival was recorded for 20μg/l PCN congeners. On the other hand, embryos exposed to 30 and 50μg/l PCNs showed a significant reduction in survival rate. No developmental defects occurred with any of the congeners at the 20μg/l concentration. However, starting with 30μg/l, weak to very pronounced abnormalities (Lordosis, Kyphosis, disrupted axial body, tail defect, reduced heartbeat, and abnormal hatching) were observed depending on the congener and the concentration used. PCN66 has been shown to be more embryotoxic congener than PCN50. This demonstration of zebrafish responsiveness to PCNs early life stage toxicity coupled with the considerable information on developmental biology and genetics of zebrafish provides a foundation for future investigations into the mechanism of PCNs developmental toxicity.
本研究考察了 PCN 同系物（PCN50 和 PCN66）对斑马鱼胚胎的影响。通过溶解 0.08%丙酮，将不同浓度（20、30 和 50μg/l）的相应同系物暴露于胚胎中。研究了以下终点（死亡率、孵化率、畸形和心率）。20μg/l PCN 同系物对生存没有影响。另一方面，暴露于 30 和 50μg/l PCN 的胚胎存活率显著降低。在 20μg/l 浓度下，任何同系物都没有发生发育缺陷。然而，从 30μg/l 开始，根据同系物和浓度的不同，观察到弱至非常明显的畸形（脊柱后凸、脊柱前凸、轴向身体中断、尾巴缺陷、心跳减少和异常孵化）。PCN66 被证明比 PCN50 更具有胚胎毒性。这一证明表明斑马鱼对 PCN 早期生命阶段毒性的反应性，加上对斑马鱼发育生物学和遗传学的大量信息，为未来研究 PCN 发育毒性的机制提供了基础。
