Key Laboratory of Yangtze River Water Environment, Ministry of Education, College of Environmental Science and Engineering, Tongji University, Shanghai, 200092, China.
Environ Sci Pollut Res Int. 2016 Sep;23(18):18832-41. doi: 10.1007/s11356-016-7092-4. Epub 2016 Jun 18.
Zebrafish (Danio rerio) embryos and larvae were selected to investigate the potential risk and aquatic toxicity of a widely used pharmaceutical, naproxen. The acute toxicity of naproxen to embryos and larvae was measured, respectively. The histopathology was investigated in the liver of zebrafish larvae after 8-day embryo-larvae exposure to naproxen. The values of 96-h LC50 were 115.2 mg/L for embryos and 147.6 mg/L for larvae, indicating that zebrafish embryos were more sensitive than larvae to naproxen exposure. Large suites of symptoms were induced in zebrafish (D. rerio) early life stages by different dosages of naproxen, including hatching inhibition, lower heart rate, and morphological abnormalities. The most sensitive sub-lethal effect caused by naproxen was pericardial edema, the 72-h EC50 values of which for embryos and larvae were 98.3 and 149.0 mg/L, respectively. In addition, naproxen-treated zebrafish larvae exhibited histopathological liver damage, including swollen hepatocytes, vacuolar degeneration, and nuclei pycnosis. The results indicated that naproxen is a potential threat to aquatic organisms.
斑马鱼（Danio rerio）胚胎和幼鱼被选来研究一种广泛使用的药物萘普生的潜在风险和水生毒性。分别测定了萘普生对胚胎和幼鱼的急性毒性。在胚胎-幼鱼暴露于萘普生 8 天后，研究了斑马鱼幼鱼肝的组织病理学变化。胚胎的 96 小时半数致死浓度（LC50）值为 115.2 mg/L，幼鱼的 LC50 值为 147.6 mg/L，表明斑马鱼胚胎对萘普生暴露比幼鱼更敏感。不同剂量的萘普生会引起斑马鱼（D. rerio）早期生活阶段的一系列症状，包括孵化抑制、心率降低和形态异常。萘普生引起的最敏感的亚致死效应是心包水肿，其胚胎和幼鱼的 72 小时半数有效浓度（EC50）值分别为 98.3 和 149.0 mg/L。此外，萘普生处理的斑马鱼幼鱼表现出肝组织病理学损伤，包括肝细胞肿胀、空泡变性和核固缩。结果表明，萘普生对水生生物是一种潜在的威胁。
