Biochemistry Laboratory, UR03/ES08, Human Nutrition and Metabolic Disorders, USCR Mass Spectrometry, Faculty of Medicine of Monastir, Monastir, Tunisia.
Nutrition. 2012 Jan;28(1):81-91. doi: 10.1016/j.nut.2011.02.009. Epub 2011 Jul 23.
We examined the effects of extra virgin olive oil (EVOO) and its hydrophilic and lipophilic fractions on serum lipids, oxidative stress, and morphologic and functional liver damages induced by 2,4-diclorophenoxyacetic acid (2,4-D).
Male Wistar rats were divided randomly into eight groups: control; 2,4-D at a dose of 5 mg/kg of body weight (2,4-D); 2,4-D plus EVOO (2,4-D/EVOO); 2,4-D plus the hydrophilic fraction (2,4-D/OOHF); 2,4-D plus the lipophilic fraction (2,4-D/OOLF); only EVOO (EVOO); only the hydrophilic fraction (OOHF); and only the lipophilic fraction (OOLF). These components were administered daily by gavage for 4 wk.
A hepatic architecture aberration, increased activities of aspartate and alanine aminotransferase enzymes, total and low-density lipoprotein cholesterol, and malondialdehyde (MDA) level, and a decreased antioxidant defense system were observed in the 2,4-D group. The administration of EVOO restored the damage caused by 2,4-D by a significant decrease of plasma total and low-density lipoprotein levels and a moderate increase of high-density lipoprotein cholesterol. The 2,4-D/OOHF group exhibited a pronounced enhancement of the antioxidant defense system by an increase of superoxide dismutase, catalase, and glutathione peroxidase levels and a decrease of plasma and liver MDA levels. However, less improvement in the liver histoarchitecture and antioxidant status was observed in rats supplemented with OOLF diet, despite its richness in α-tocopherol.
Extra virgin olive oil may be a potential functional food source of antioxidants than can decrease the frequency of cardiovascular diseases and liver damage.
研究特级初榨橄榄油（EVOO）及其亲水性和疏水性部分对 2,4-二氯苯氧乙酸（2,4-D）引起的血清脂质、氧化应激以及形态和功能肝损伤的影响。
雄性 Wistar 大鼠随机分为 8 组：对照组；2,4-D 剂量为 5mg/kg 体重（2,4-D）；2,4-D+EVOO（2,4-D/EVOO）；2,4-D+亲水性部分（2,4-D/OOHF）；2,4-D+疏水性部分（2,4-D/OOLF）；仅 EVOO（EVOO）；仅亲水性部分（OOHF）；仅疏水性部分（OOLF）。这些成分通过灌胃每天给药 4 周。
在 2,4-D 组观察到肝组织结构异常、天冬氨酸和丙氨酸转氨酶酶活性、总胆固醇和低密度脂蛋白胆固醇以及丙二醛（MDA）水平升高，抗氧化防御系统降低。EVOO 的给药通过显著降低血浆总胆固醇和低密度脂蛋白水平和适度增加高密度脂蛋白胆固醇来恢复 2,4-D 引起的损伤。2,4-D/OOHF 组通过增加超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶水平以及降低血浆和肝脏 MDA 水平来显著增强抗氧化防御系统。然而，尽管 OOLF 饮食富含α-生育酚，但在补充 OOLF 饮食的大鼠中，肝组织形态和抗氧化状态的改善较少。
特级初榨橄榄油可能是一种潜在的抗氧化功能食品来源，可以降低心血管疾病和肝损伤的频率。
