Nano J L, Cefai D, Rampal P
Laboratoire de Gastroentérologie et de Nutrition, U.E.R. de Médecine, Nice, France.
Alcohol Clin Exp Res. 1990 Feb;14(1):32-7. doi: 10.1111/j.1530-0277.1990.tb00442.x.
The effect of exposure of an intestinal epithelial cell line to various concentrations of ethanol [217 mM (1%) to 652 mM (3%)] during 24, 48, and 72 hr was investigated in vitro using a rat intestinal epithelial cell line (IRD 98). Incubation of these cells in the presence of ethanol significantly decreased cell growth. This inhibition was accompanied by a strong increase in cellular protein. Stimulation of specific disaccharidases, gamma-glutamyl transferase, and aminopeptidase activities by ethanol was dose- and time-dependent. Ethanol induces a change in the relative proportions of the different lipid classes synthesized; triglycerides, fatty acids, and cholesterol esters were preferentially synthethysed. Our findings show that cell lines are good models for investigation of the effects of ethanol, and that alcohol considerably modifies the functions of intestinal epithelial cells.
使用大鼠肠道上皮细胞系（IRD 98）在体外研究了肠道上皮细胞系在24小时、48小时和72小时内暴露于不同浓度乙醇[217 mM（1%）至652 mM（3%）]的影响。在乙醇存在下培养这些细胞会显著降低细胞生长。这种抑制伴随着细胞蛋白质的强烈增加。乙醇对特定二糖酶、γ-谷氨酰转移酶和氨肽酶活性的刺激呈剂量和时间依赖性。乙醇会诱导合成的不同脂质类别的相对比例发生变化；甘油三酯、脂肪酸和胆固醇酯被优先合成。我们的研究结果表明，细胞系是研究乙醇作用的良好模型，并且酒精会显著改变肠道上皮细胞的功能。
