Pösö R, Pösö H, Väänänen H, Salaspuro M
Adv Exp Med Biol. 1980;132:551-60. doi: 10.1007/978-1-4757-1419-7_57.
The effects of ethanol on DNA and protein synthesis of regenerating rat liver has been studied. A single dose of ethanol, when given either before, at the time or 1 h after the partial hepatectomy significantly inhibited the accelerated synthesis of ornithine decarboxylase protein which was used as a marker of protein synthesis. Ethanol seemed to inhibit the synthesis of ornithine decarboxylase at transcriptional level of protein synthesis and the inhibition appeared to be directly due to the presence of ethanol molecules. When rats were chronically fed with ethanol-containing liquid diet also the total protein synthesis was inhibited during the two first days of regeneration. In this experiment also the rate of DNA synthesis was greatly inhibited by ethanol treatment. The results show that the ethanol-induced inhibition of the synthesis of tissue macromolecules is ideally demonstrated in regenerating rat liver. Furthermore they suggest that this effect may be of importance in the progression of alcoholic liver injury.
已经研究了乙醇对再生大鼠肝脏DNA和蛋白质合成的影响。在部分肝切除术前、术时或术后1小时给予单剂量乙醇，可显著抑制用作蛋白质合成标志物的鸟氨酸脱羧酶蛋白的加速合成。乙醇似乎在蛋白质合成的转录水平抑制鸟氨酸脱羧酶的合成，这种抑制似乎直接归因于乙醇分子的存在。当大鼠长期喂食含乙醇的液体饲料时，在再生的前两天总蛋白质合成也受到抑制。在该实验中，乙醇处理也极大地抑制了DNA合成速率。结果表明，乙醇诱导的组织大分子合成抑制在再生大鼠肝脏中得到了理想的证明。此外，这些结果表明这种作用可能在酒精性肝损伤的进展中具有重要意义。
