Department of Virology and A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow 119991, Russia.
Nucleic Acids Res. 2011 Nov 1;39(20):8869-80. doi: 10.1093/nar/gkr594. Epub 2011 Jul 23.
Fibrillarin, one of the major proteins of the nucleolus, plays several essential roles in ribosome biogenesis including pre-rRNA processing and 2'-O-ribose methylation of rRNA and snRNAs. Recently, it has been shown that fibrillarin plays a role in virus infections and is associated with viral RNPs. Here, we demonstrate the ability of recombinant fibrillarin 2 from Arabidopsis thaliana (AtFib2) to interact with RNAs of different lengths and types including rRNA, snoRNA, snRNA, siRNA and viral RNAs in vitro. Our data also indicate that AtFib2 possesses two RNA-binding sites in the central (138-179 amino acids) and C-terminal (225-281 amino acids) parts of the protein, respectively. The conserved GCVYAVEF octamer does not bind RNA directly as suggested earlier, but may assist with the proper folding of the central RNA-binding site.
核仁主要蛋白之一的纤维蛋白在核糖体生物发生中发挥着几个重要作用，包括前 rRNA 加工和 rRNA 和 snRNA 的 2'-O-核糖甲基化。最近，已经表明纤维蛋白在病毒感染中起作用，并与病毒 RNP 相关。在这里，我们证明了来自拟南芥（AtFib2）的重组纤维蛋白 2 的能力，以与不同长度和类型的 RNA 相互作用，包括 rRNA、snoRNA、snRNA、siRNA 和病毒 RNA。我们的数据还表明，AtFib2 在蛋白质的中央（138-179 个氨基酸）和 C 末端（225-281 个氨基酸）部分分别具有两个 RNA 结合位点。如前所述，保守的 GCVYAVEF 八聚体并不直接与 RNA 结合，而是可能有助于中央 RNA 结合位点的正确折叠。
