Department of Oncology, First Affiliated Clinic College of Harbin Medical University, Harbin, China.
Cancer Biol Ther. 2011 Oct 1;12(7):610-6. doi: 10.4161/cbt.12.7.16302.
The relationship between IL-17A and cancer, whether beneficial or antagonistic, continues to be a controversial issue. In this study, effects of IL-17A on lung adenocarcinoma were investigated using lung cancer cell lines, 95D and 95C. In the presence or absence of IL-17A, cell proliferation and VEGF secretion were detected. Effects of IL-17A on capillary networks and process of angiogenesis were also evaluated. In vivo, the level of IL-17A was assayed in the serum of lung adenocarcinoma patients. At the same time, slices of adenocarcinoma tissue were analyzed for expression of IL-17A, its receptor (IL-17RA), VEGF, CD4(+)-IL-17A+ cells and CD8(+)-IL-17A+ cells by immunohistochemistry and immunofluorescence assays. IL-17A did not have effect on the proliferation of 95D or 95C cells, however, the elevated expression of VEGF in supernatant of 95D or 95C cells was found to be IL-17A concentration-dependent. Supernatants from 95D or 95C cells treated with IL-17A could obviously facilitate angiogenesis, compared with IL-17A absence group (P < 0.01). Higher levels of IL-17A were detected in serum of patients with lung adenocarcinoma than healthy controls (P < 0.001). Higher positive expressions of IL-17A, IL-17RA and VEGF were confirmed in lung adenocarcinoma lesion tissues compared to pericancerous normal tissues (P < 0.001). Tnc17 cells, as well as Th17 cells were found in adenocarcinoma tissue, indicating a potential role of these cells in disease. In summary, IL-17A might affect lung adenocarcinoma by promoting angiogenesis, while the role of Tnc17 cells or Th17 cells remains to be elucidated.
IL-17A 与癌症之间的关系，无论是有益的还是拮抗的，仍然是一个有争议的问题。在这项研究中，使用肺癌细胞系 95D 和 95C 研究了 IL-17A 对肺腺癌的影响。在存在或不存在 IL-17A 的情况下，检测细胞增殖和 VEGF 分泌。还评估了 IL-17A 对毛细血管网络和血管生成过程的影响。在体内，检测了肺腺癌患者血清中 IL-17A 的水平。同时，通过免疫组织化学和免疫荧光分析分析腺癌组织切片中 IL-17A、其受体（IL-17RA）、VEGF、CD4（+）-IL-17A+细胞和 CD8（+）-IL-17A+细胞的表达。IL-17A 对 95D 或 95C 细胞的增殖没有影响，然而，发现上清液中 VEGF 的高表达与 IL-17A 浓度呈依赖性。与无 IL-17A 组相比，用 IL-17A 处理的 95D 或 95C 细胞的上清液明显促进血管生成（P < 0.01）。与健康对照组相比，肺腺癌患者血清中 IL-17A 水平较高（P < 0.001）。与癌旁正常组织相比，肺腺癌病变组织中 IL-17A、IL-17RA 和 VEGF 的阳性表达更高（P < 0.001）。在腺癌组织中发现了 Tnc17 细胞和 Th17 细胞，表明这些细胞在疾病中可能发挥作用。总之，IL-17A 可能通过促进血管生成来影响肺腺癌，而 Tnc17 细胞或 Th17 细胞的作用仍有待阐明。
