Liu Yanbo, Sun Xuemei, Zhao Xiaohui, An Liping, Wang Zhuxing, Jiang Jing, Shen Weigao, Yang Xueliang, Sun Ying
The Clinical Immunology Center, Beihua University, Jilin, Jilin, 132013, China.
The Clinical Immunology Center, Beihua University, Jilin, Jilin, 132013, China.
Pathol Res Pract. 2018 Apr;214(4):482-491. doi: 10.1016/j.prp.2018.03.011. Epub 2018 Mar 7.
The research aimed to investigate secretion, expression and location of IL-17 relative ligands, IL-17 relative receptors, infiltrating inflammatory cells and parenchymal structural cells in colorectal cancer (CRC) compared with ulcerative colitis (UC) and benign hyperplastic polyp. 29 human intestinal tissues with CRC, 17 with UC and 7 with polyp were stained using immunohistochemistry to evaluate immunoreactivity for IL-17 family relative ligands including IL-17A, E, F and their respective relative receptors such as IL-17RA, IL-17RB and IL-17RC. At the same time the infiltration of inflammatory cells including lymphocytes, phagocytes, mast cells and neutrophils and parenchymal structural cell changes involving vascular endothelial cells and CD90 fibroblast cells were also evaluated using the same methods The immunoreactivity or positive inflammatory cells of all the sections were analyzed using professional image analysis software to determine statistical significance. The immunoreactivity for IL-17A, IL-17RA, IL-17E, IL-17RB and IL-17F showed significant decrease in CRC tissue when compared to UC (p = 0.00001. respectively). The reduction of above IL-17 relative ligands and receptors was accompanied by an obvious decrease in the number of infiltrating neutrophils and mast cells in CRC (p = 0.00001 and p = 0.007, respectively) but accompanied by a marked increase of CD31 blood vessels (p = 0.001). The immunoreactivity of IL-17A, IL-17RA, IL-17E, IL-17RB and IL-17F and the numbers of infiltrating neutrophils and mast cells showed significant decrease in CRC tissues when compared to those in polyp (p < 0.05). In contrast, the immunoreactivity of IL-17RC and the numbers of CD3 1ymphocytes were elevated in CRC when compared with those in polyp (p = 0.0001, p = 0.007, respectively). In CRC tissues, positive correlations between IL-17A, IL-17RA with CD68 macrophages were observed respectively (r = 0.621, p = 0.0001; r = 0.75, p = 0.0001). IL-17 cytokine family including ligands and their corresponding receptors were secreted and expressed by infiltrating inflammatory cells. Not only infiltrating lymphocytes but also increased blood endothelial cells were relative significantly to genesis and progression of CRC.
本研究旨在调查与溃疡性结肠炎（UC）和良性增生性息肉相比，白细胞介素-17相关配体、白细胞介素-17相关受体、浸润性炎症细胞和实质结构细胞在结直肠癌（CRC）中的分泌、表达和定位情况。采用免疫组织化学方法对29例结直肠癌患者、17例溃疡性结肠炎患者和7例息肉患者的肠道组织进行染色，以评估白细胞介素-17家族相关配体（包括白细胞介素-17A、E、F）及其各自相关受体（如白细胞介素-17RA、白细胞介素-17RB和白细胞介素-17RC）的免疫反应性。同时，采用相同方法评估包括淋巴细胞、吞噬细胞、肥大细胞和中性粒细胞在内的炎症细胞浸润以及涉及血管内皮细胞和CD90成纤维细胞的实质结构细胞变化。使用专业图像分析软件分析所有切片的免疫反应性或阳性炎症细胞，以确定统计学意义。与UC相比，结直肠癌组织中白细胞介素-17A、白细胞介素-17RA、白细胞介素-17E、白细胞介素-17RB和白细胞介素-17F的免疫反应性显著降低（p分别为0.00001）。上述白细胞介素-17相关配体和受体的减少伴随着结直肠癌中浸润性中性粒细胞和肥大细胞数量的明显减少（p分别为0.00001和0.007），但伴随着CD31血管的显著增加（p = 0.001）。与息肉相比，结直肠癌组织中白细胞介素-17A、白细胞介素-17RA、白细胞介素-17E、白细胞介素-17RB和白细胞介素-17F的免疫反应性以及浸润性中性粒细胞和肥大细胞的数量显著减少（p < 0.05）。相比之下，与息肉相比，结直肠癌中白细胞介素-17RC的免疫反应性和CD3 +淋巴细胞的数量升高（p分别为0.0001和0.007）。在结直肠癌组织中，分别观察到白细胞介素-17A、白细胞介素-17RA与CD68巨噬细胞之间存在正相关（r = 0.621，p = 0.0001；r = 0.75，p = 0.0001）。包括配体及其相应受体在内的白细胞介素-17细胞因子家族由浸润性炎症细胞分泌和表达。不仅浸润性淋巴细胞，而且增加的血管内皮细胞与结直肠癌的发生和进展也显著相关。
