Institute of Photonic Technologies, Albert-Einstein-Straße 9, 07745 Jena, Germany.
Analyst. 2011 Sep 21;136(18):3686-93. doi: 10.1039/c0an00956c. Epub 2011 Jul 25.
The introduction of carbon-deuterium (C-D) bonds into drug compounds by organic synthesis is a non-invasive labelling approach, which does not alter the chemical and physiological properties of the drug itself. C-deuterated drugs exhibit characteristic vibrational signatures in the C-D stretching region around 2100-2300 cm(-1), which avoids spectral interference with contributions from a complex biological environment. In this paper, the quantitative detection of C-deuterated drugs by Raman microspectroscopy and single-band CARS microscopy is examined. Concentration-dependent studies on drugs with aliphatic and aromatic C-D moieties were performed in a two-channel microfluidic chip, using the corresponding non-deuterated (C-H) isotopologues as an internal reference.
通过有机合成在药物化合物中引入碳氘（C-D）键是一种非侵入性标记方法，不会改变药物本身的化学和生理性质。C-氘化药物在 2100-2300cm(-1) 左右的 C-D 伸缩区域表现出特征振动特征，避免了与复杂生物环境贡献的光谱干扰。本文研究了拉曼微光谱和单频 CARS 显微镜定量检测 C-氘化药物的方法。在双通道微流控芯片中，使用相应的非氘化（C-H）同位素作为内标，对具有脂肪族和芳香族 C-D 部分的药物进行了浓度依赖性研究。
