Fomenko N V, Borgoiakov V Iu, Panov V V
Mol Gen Mikrobiol Virusol. 2011(2):12-7.
The definition and molecular typing of Borrelia miyamotoi transmitted by the Ixodes persuccatus ticks was based on the partial sequencing of the 16S rRNA, p66, and glpQ genes. All the B. miyamotoi analyzed sequences of the 16S rRNA, glpQ, and p66 gene fragments from I. persulcatus were identical and had 99.9-100% similarity to corresponding genes of the B. miyamotoi strain FR64b isolated in Japan. The analyzed amino acid sequences revealed that the 66 protein B. miyamotoi in the site corresponding to the surface-exposed domain contained considerable difference from the Borrelia hermsii, the typical member tick-born relapsing fever, as from Borrelia lonestari transmitted by the Ixodes ticks.
通过全沟硬蜱传播的宫本疏螺旋体的定义和分子分型基于16S rRNA、p66和glpQ基因的部分测序。对所有全沟硬蜱分离的宫本疏螺旋体的16S rRNA、glpQ和p66基因片段进行分析，其序列相同，与日本分离的宫本疏螺旋体菌株FR64b的相应基因具有99.9 - 100%的相似性。分析的氨基酸序列显示，宫本疏螺旋体66蛋白在对应于表面暴露结构域的位点与典型的蜱传回归热赫氏疏螺旋体以及由硬蜱传播的孤独疏螺旋体存在显著差异。
