用于检测北美野外采集的硬蜱若虫中广义伯氏疏螺旋体和广义宫本疏螺旋体的三种多重检测方法。
Three multiplex assays for detection of Borrelia burgdorferi sensu lato and Borrelia miyamotoi sensu lato in field-collected Ixodes nymphs in North America.
作者信息
Ullmann A J, Gabitzsch E S, Schulze T L, Zeidner N S, Piesman J
机构信息
Division of Vector-Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, CO 80522, USA.
出版信息
J Med Entomol. 2005 Nov;42(6):1057-62. doi: 10.1093/jmedent/42.6.1057.
Two hundred fifty New Jersey field-collected Ixodes scapularis Say ticks and 17 Colorado Ixodes spinipalpis Hadwen & Nuttall ticks were tested using three separate multiplex real-time polymerase chain reaction (PCR) assays. One assay targets the rrs-rrlA IGS region of Borrelia spp. to detect Borrelia burgdorferi sensu lato (s.l.) and Borrelia miyamotoi s.l. The second assay targets the ospA region of B. burgdorferi s.l. to detect B. burgdorferi sensu stricto (s.s.), Borrelia bissettii, and Borrelia andersonii. The final assay targets the glpQ region of B. miyamotoi s.l. to differentiate B. miyamotoi LB-2001 and Borrelia lonestari. A testing scheme combining these tests yielded 18% of tested I. scapularis ticks surveyed from New Jersey positive for B. burgdorferi s.s., 3.2% I. scapularis ticks positive for B. miyamotoi LB-2001, and 41.2% I. spinipalpis ticks positive for B. bissettii surveyed from Colorado.
使用三种独立的多重实时聚合酶链反应（PCR）检测方法，对250只新泽西州野外采集的肩突硬蜱（Ixodes scapularis Say）和17只科罗拉多州的斯氏硬蜱（Ixodes spinipalpis Hadwen & Nuttall）进行了检测。一种检测方法针对疏螺旋体属（Borrelia spp.）的rrs-rrlA间隔区，以检测广义伯氏疏螺旋体（Borrelia burgdorferi sensu lato，s.l.）和米亚托疏螺旋体（Borrelia miyamotoi s.l.）。第二种检测方法针对狭义伯氏疏螺旋体（B. burgdorferi sensu stricto，s.s.）的ospA区域，以检测狭义伯氏疏螺旋体、比氏疏螺旋体（Borrelia bissettii）和安德森疏螺旋体（Borrelia andersonii）。最后一种检测方法针对米亚托疏螺旋体的glpQ区域，以区分米亚托疏螺旋体LB-2001和朗斯塔里疏螺旋体（Borrelia lonestari）。将这些检测方法结合起来的检测方案显示，在新泽西州采集的肩突硬蜱中，18%检测出狭义伯氏疏螺旋体呈阳性，3.2%的肩突硬蜱检测出米亚托疏螺旋体LB-2001呈阳性，在科罗拉多州采集的斯氏硬蜱中，41.2%检测出比氏疏螺旋体呈阳性。
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