College of Veterinary Medicine, Northeast Agricultural University, Changjiang Road No.600, Harbin 150030, Heilongjiang Province, China.
Second Affiliated Hospital of Inner Mongolia University for the Nationalities, Inner Mongolia General Forestry Hospital, Yakeshi, Inner Mongolia Autonomous Region, China.
Acta Trop. 2021 May;217:105857. doi: 10.1016/j.actatropica.2021.105857. Epub 2021 Feb 11.
Borrelia miyamotoi is a relapsing fever spirochete that can cause chills, fatigue, headache, myalgia, arthralgia, and even meningitis, damaging human health. B. miyamotoi has a wide distribution since its discovery in Ixodes persulcatus in 1994. The human B. miyamotoi disease was first described in Russia in 2011. However, the epidemiological information in China is limited. Here, we report the molecular detection of B. miyamotoi in the northeast of Inner Mongolia, China. A total of 774 adult ticks and 771 blood samples of patients were collected, from April 2017 to August 2019 in the northeast of Inner Mongolia, and tested for B. miyamotoi using real time-PCR. Gene sequences of 16S rRNA, fla, and glpQ were obtained to reconstruct the phylogenetic relationship of B. miyamotoi from humans and ticks. The results showed the total prevalence of B. miyamotoi in ticks was 1.3% of 774 ticks, with rates of 2.6% in I. persulcatus, 0.78% in Dermacentor nuttalli, 1.3% in D. silvarum, and 0.4% in Haemaphysalis longicornis. Thirteen (1.7%) patients were confirmed as positive for B. miyamotoi. Patients were mainly 50-60-years old and had a history of tick contact. They presented flu-like symptoms, including fever, headache, poor spirit, dizziness, nausea, vomiting, hypodynamic, chest distress, and myalgia. Phylogenetic analysis showed that the B. miyamotoi in the present study belonged to the Siberian type, distinct from European and American types and the I. ovatus isolate from Japan. This is the first report of B. miyamotoi detection in both ticks and humans in the northeast of Inner Mongolia, China, indicating B. miyamotoi is present in the area. These findings suggest that people have a risk of infection with B. miyamotoi in this region, where it should be included the differential diagnosis of tick-borne diseases.
伯氏疏螺旋体是一种回归热螺旋体，可引起寒战、疲劳、头痛、肌痛、关节痛，甚至脑膜炎，损害人类健康。自 1994 年在日本璃眼蜱中发现以来，伯氏疏螺旋体分布广泛。2011 年，俄罗斯首次描述了人类伯氏疏螺旋体病。然而，中国的流行病学信息有限。在这里，我们报告了在中国内蒙古东北部对伯氏疏螺旋体的分子检测。共采集 2017 年 4 月至 2019 年 8 月内蒙古东北部地区 774 只成年蜱和 771 份患者血液样本，采用实时 PCR 检测伯氏疏螺旋体。获得 16S rRNA、fla 和 glpQ 基因序列，从人和蜱中重建伯氏疏螺旋体的系统发育关系。结果显示，蜱总伯氏疏螺旋体阳性率为 774 只蜱的 1.3%，其中璃眼蜱为 2.6%，全沟硬蜱为 0.78%，森林革蜱为 1.3%，长角血蜱为 0.4%。13 例（1.7%）患者被确认为伯氏疏螺旋体阳性。患者主要为 50-60 岁，有蜱接触史。他们表现出流感样症状，包括发热、头痛、精神不振、头晕、恶心、呕吐、乏力、胸闷、肌痛。系统发育分析显示，本研究中的伯氏疏螺旋体属于西伯利亚型，与欧洲和美洲型以及日本的日本璃眼蜱分离株不同。这是中国内蒙古东北部蜱和人类中伯氏疏螺旋体检测的首次报告，表明该地区存在伯氏疏螺旋体。这些发现表明，该地区的人有感染伯氏疏螺旋体的风险，应将其纳入蜱传疾病的鉴别诊断。
