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[利用果蝇奥雷纳染色体中心的DNA文库对黑腹果蝇假滋养细胞细胞核中多线染色体的组织进行研究]

[Study of the organization of polytene chromosomes in the nuclei of Drosophila melanogaster pseudonurse cells with the use of the DNA library of the Drosophila orena chromocenter].

作者信息

Stegniĭ V N, Nemirovich-Danchenko N M, Bagdalova A D

出版信息

Genetika. 2011 May;47(5):697-702.

Abstract

The location of the Drosophila orena chromocenter in polytene chromosomes of pseudonurse cells of the D. melanogaster ovaries (the otu11 mutation) and salivary glands has been studied. Numerous sites of location of the D. orena chromocenter DNA have been found throughout the length of D. melanogaster chromosomes. The specific distribution of the binding sites for the DNA probe has made it possible to identify chromosomes and analyze their mutual positions in the three-dimensional space of the nuclei of pseudonurse cells. The mutual positions of chromosomes have been found to vary, the pericentromeric regions of different chromosomes differing from one another in associative ratios.

摘要

对黑腹果蝇卵巢假滋养细胞(otu11突变)和唾液腺多线染色体中果蝇orena染色中心的位置进行了研究。在黑腹果蝇染色体全长上发现了多个果蝇orena染色中心DNA的定位位点。DNA探针结合位点的特定分布使得识别染色体并分析它们在假滋养细胞核三维空间中的相互位置成为可能。已发现染色体的相互位置有所不同,不同染色体的着丝粒周围区域在关联比率上彼此不同。

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