Mal'ceva N I, Gyurkovics H, Zhimulev I F
Laboratory of Molecular Cytogenetics, Siberian Division of Russian Academy of Sciences, Novosibirsk.
Chromosome Res. 1995 May;3(3):191-200. doi: 10.1007/BF00710713.
Polytene chromosomes of good cytological quality from pseudonurse cells (PNCs) of fs(2)B and otu11 mutants were obtained, photomaps for otu11 mutants were constructed and the general characteristics of polytene chromosomes from salivary glands (SGs) and PNCs were compared. Three conditions were found to improve the cytological quality of PNC chromosomes: temperature below 18 degrees C, a protein-rich medium and presence of the Y-chromosome. Detailed comparison of the chromosome banding pattern from SGs and PNCs has shown only minor differences between them. The frequency of asynapsis appeared to be 10 times higher for PNC chromosomes. Despite previous reports, features such as breaks and ectopic contacts turned out to be also typical for PNC chromosomes, but with remarkably lower frequencies.
从fs(2)B和otu11突变体的假滋养细胞（PNC）中获得了细胞学质量良好的多线染色体，构建了otu11突变体的光图谱，并比较了唾液腺（SG）和PNC多线染色体的一般特征。发现三种条件可提高PNC染色体的细胞学质量：温度低于18摄氏度、富含蛋白质的培养基和Y染色体的存在。SG和PNC染色体带型的详细比较显示它们之间只有微小差异。PNC染色体的联会消失频率似乎高10倍。尽管有先前的报道，但断裂和异位接触等特征结果也为PNC染色体所特有，但频率明显较低。
