Sharakhov I V, Vasserlauf I E, Stegniĭ V N
Genetika. 1997 Feb;33(2):189-95.
Certain aspects of the polytene chromosome attachment to the nuclear envelope (NE) of ovarian pseudonurse cells (PNC) in the mutant strains otu11 and fs(2) B of Drosophila melanogaster were studied. In contrast to salivary a local chromocenter and Rable-orientation of chromosomes were shown to be missing from the PNC nuclei. The pericentric regions were scattered in the nuclear space and were obliged to attach to the NE by specific loci of heterochromatin. These loci were assigned to the regions 20CD and 41, and the proximal regions 81 and 101 of the cytological map of secondary polytene chromosomes. Each of the loci, which was obliged to attach to the NE, had a specific morphology that was similar in the otu11 and fs(2)B strains. PNC were shown to resemble normal ovarian nurse cells of D. melanogaster in the position of the polytene chromosomes and their attachment to the NE.
对黑腹果蝇突变品系otu11和fs(2)B中卵巢假滋养细胞(PNC)的多线染色体附着于核膜(NE)的某些方面进行了研究。与唾液腺不同,PNC细胞核中未显示局部染色中心和染色体的拉布尔定向。着丝粒周围区域散布在核空间中,并通过异染色质的特定位点附着于核膜。这些位点被定位到次生多线染色体细胞学图谱的20CD和41区,以及近端的81和101区。每个必须附着于核膜的位点都有特定的形态,在otu11和fs(2)B品系中相似。PNC在多线染色体的位置及其与核膜的附着方面与黑腹果蝇正常的卵巢滋养细胞相似。