Department of Chemistry, National University of Singapore, Singapore 117543.
Org Lett. 2011 Aug 19;13(16):4160-3. doi: 10.1021/ol201430x. Epub 2011 Jul 25.
A small molecule-assisted protein labeling strategy based on a mutant SNAP-Tag (mSNAP) and its FRET substrate pairs has been developed. Both one- and two-photon fluorescence microscopic experiments were successfully demonstrated in living cells.
已经开发出一种基于突变型 SNAP 标签(mSNAP)及其 FRET 底物对的小分子辅助蛋白标记策略。在活细胞中成功地进行了单光子和双光子荧光显微镜实验。