Department of Medical Biotechnology, College of Biomedical Science, Kangwon National University, Chuncheon, Gangwon-Do 200-701, Republic of Korea.
Environ Toxicol Pharmacol. 2011 Jan;31(1):42-50. doi: 10.1016/j.etap.2010.09.004. Epub 2010 Sep 15.
Duchesnea indica (Rosaceae family) is herb used extensively in traditional Chinese medicine. In this study we investigated its protective activity against hydrogen peroxide (H(2)O(2))-induced cytotoxicity in human skin fibroblast (CCD-986Sk) cells and 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced H(2)O(2) in the skin of hairless mice. Pretreatment of CCD-986Sk cells with methanolic extract of D. indica (DIM) improved the cell viability, enhanced activity of catalase, and decreased the leakage of lactate dehydrogenase (LDH) and the levels of malondialdehyde (MDA) and intracellular reactive oxygen species (ROS) in H(2)O(2) injured cells. Furthermore, DIM inhibited cell apoptosis and Bax expression induced by H(2)O(2). In addition, the level of H(2)O(2) stimulated by TPA was decreased by DIM in the skin of hairless mice. These results suggest that DIM offers protection against oxidative stress in vitro and in vivo, and this ability suggests potential use for protection against oxidation-induced skin damage.
藤莓（蔷薇科）是一种广泛应用于传统中药的草本植物。在这项研究中，我们研究了其对人皮肤成纤维细胞（CCD-986Sk）和 12-O-十四烷酰佛波醇-13-乙酸酯（TPA）诱导的过氧化氢（H2O2）引起的无毛小鼠皮肤损伤的保护活性。用藤莓的甲醇提取物（DIM）预处理 CCD-986Sk 细胞，可提高细胞活力，增强过氧化氢酶活性，并降低过氧化氢损伤细胞中乳酸脱氢酶（LDH）和丙二醛（MDA）的漏出以及细胞内活性氧（ROS）的水平。此外，DIM 抑制了 H2O2 诱导的细胞凋亡和 Bax 表达。此外，在无毛小鼠的皮肤中，DIM 还降低了 TPA 刺激产生的 H2O2 水平。这些结果表明，DIM 提供了体外和体内抗氧化应激的保护，这种能力表明其在预防氧化诱导的皮肤损伤方面具有潜在用途。
