Radiation and Cancer Biology Laboratory, Department of Zoology, University of Rajasthan, Jaipur 302004, Rajasthan, India.
Environ Toxicol Pharmacol. 2011 May;31(3):427-35. doi: 10.1016/j.etap.2011.02.004. Epub 2011 Feb 18.
The present study was undertaken to determine the optimum effective dose, dose reduction factor (DRF) and possible mechanism of action of Aloe gel. Three different doses of gel (250, 500 and 750 mg/kg body weight) were tested against 8 Gy induced damage in Swiss albino mice. A dose of 750 mg/kg body weight of Aloe was found the most effective while, 250 mg/kg body weight was the least effective in providing protection, as observed in the form of higher concentrations of blood GSH and vitamin C and lower level of serum LPO than irradiated animals at 1h post irradiation and higher percent of survivors up to day 30 post irradiation. Treatment of mice with Aloe before irradiation with different doses of gamma radiation (6-12 Gy) delayed the onset and reduced the severity of signs of radiation sickness. The LD(50/30) was calculated as 6.77 and 10 Gy for untreated irradiated and Aloe treated irradiated animals, respectively and its dose reduction factor was also calculated as 1.47. Aloe gel scavenged the free radicals, DPPH•, ABTS(+•) and NO in a concentration dependent manner in vitro and therefore, scavenging of free radicals seems to be its important mechanism of action.
本研究旨在确定芦荟凝胶的最佳有效剂量、剂量减少因子（DRF）和可能的作用机制。三种不同剂量的凝胶（250、500 和 750mg/kg 体重）在瑞士白化小鼠中进行了 8Gy 诱导损伤的测试。结果发现，750mg/kg 体重的芦荟凝胶最为有效，而 250mg/kg 体重的芦荟凝胶则最无效，这表现为在照射后 1 小时，血液 GSH 和维生素 C 浓度较高，血清 LPO 水平较低，并且在照射后 30 天内的存活率较高。在照射前用不同剂量的γ射线（6-12Gy）处理小鼠，用芦荟凝胶处理可延迟辐射病症状的出现并减轻其严重程度。未处理的照射和用芦荟处理的照射动物的 LD(50/30)分别计算为 6.77 和 10Gy，其剂量减少因子也计算为 1.47。芦荟凝胶在体外以浓度依赖的方式清除自由基 DPPH•、ABTS(+•)和 NO，因此，清除自由基似乎是其重要的作用机制。
