Gametogenesis Research Center, Institute for Basic Sciences, Kashan University of Medical Sciences, Kashan, Iran.
Department of Anatomical Sciences & Cognitive Neuroscience, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
Reprod Sci. 2024 Nov;31(11):3512-3520. doi: 10.1007/s43032-024-01680-y. Epub 2024 Aug 29.
Ovarian tissue cryopreservation is regarded as useful method for fertility preservation. This study aimed to preserve most of the follicular reserve from the destructive effects of cryoprotectant solutions and liquid nitrogen. For this purpose, 48 female NMRI mice (8 weeks old) were randomly divided into six groups: Fresh (not vitrified), Vitrification (not encapsulated), Alginate 1 (encapsulated in 1% alginate hydrogel before placing in vitrification solutions), Alginate 2 (encapsulated in 1% alginate hydrogel before placing in liquid nitrogen), Aloe vera 1 (encapsulated in Aloe vera pieces before placing in vitrification solutions), Aloe vera 2 (encapsulated in Aloe vera pieces before placing in liquid nitrogen). After vitrification and warming, the histological evaluation showed that the average number of intact primordial follicles decreased significantly in all groups compared to the Fresh group. (P < 0.05). Results of evaluating the expression of apoptosis-related genes showed that the ratio of Bax/Bcl2 and P53 significantly decreased in the Alginate 2 group compared with the vitrification group. The level of Kit gene (KIT proto-oncogeni receptor tyrosine kinase gene) expression was either the same or lower in the experimental groups than in the vitrification group, but there was no statistically significant difference. Levels of tissue nitric oxide (NO) and malondialdehyde (MDA) in Alginate groups 1 and 2 showed a significant decrease compared with the vitrification group (P < 0.05). To conclude, Encapsulation of ovaries in 1% alginate hydrogel before immersion in liquid nitrogen may reduce the damage caused by cryopreservation.
卵巢组织冷冻保存被认为是一种有用的生育力保存方法。本研究旨在从冷冻保护剂溶液和液氮的破坏性影响中保留大部分卵泡储备。为此，将 48 只雌性 NMRI 小鼠（8 周龄）随机分为六组：新鲜组（未玻璃化）、玻璃化组（未包封）、藻酸盐 1 组（包封在 1%藻酸盐水凝胶中，然后放入玻璃化溶液中）、藻酸盐 2 组（包封在 1%藻酸盐水凝胶中，然后放入液氮中）、芦荟 1 组（包封在芦荟块中，然后放入玻璃化溶液中）、芦荟 2 组（包封在芦荟块中，然后放入液氮中）。玻璃化和复温后，组织学评价显示，与新鲜组相比，所有组的完整原始卵泡数量均显著减少（P<0.05）。评估凋亡相关基因表达的结果表明，与玻璃化组相比，藻酸盐 2 组的 Bax/Bcl2 和 P53 比值显著降低。Kit 基因（Kit 原癌基因受体酪氨酸激酶基因）表达水平在实验组与玻璃化组相同或更低，但无统计学差异。藻酸盐 1 组和 2 组的组织一氧化氮（NO）和丙二醛（MDA）水平与玻璃化组相比均显著降低（P<0.05）。综上所述，将卵巢浸泡在液氮前用 1%藻酸盐水凝胶包封可能会减少冷冻保存造成的损伤。
