Laboratory of Host-Parasite Interaction Studies, ICMR-National Institute of Malaria Research, New Delhi, India.
Bio and Nanotechnology Department, Guru Jambheshwar University of Science and Technology, Haryana, India.
Front Immunol. 2020 May 14;11:609. doi: 10.3389/fimmu.2020.00609. eCollection 2020.
Blood-feeding enriched gut-microbiota boosts mosquitoes' anti- immunity. Here, we ask how alters gut-microbiota, anti- immunity, and impacts tripartite -mosquito-microbiota interactions in the gut lumen. We used a metagenomics and RNAseq strategy to address these questions. In naïve mosquitoes, and spp. are the dominant bacteria and blood-feeding leads to a heightened detection of and . A parallel RNAseq analysis of blood-fed midguts also shows the presence of transcripts. After, infected blood-meal, however, we do not detect bacterial 16S rRNA until circa 36 h. Intriguingly, the transcriptional expression of a selected array of antimicrobial arsenal cecropins 1-2, defensin-1, and gambicin remained low during the first 36 h-a time frame when ookinetes/early oocysts invaded the gut. We conclude during the preinvasive phase, outcompetes midgut-microbiota. This microbial suppression likely negates the impact of mosquito immunity which in turn may enhance the survival of . Detection of sequences matching to mosquito-associated opens a new inquiry for its exploration as an agent for "paratransgenesis-based" mosquito control.
吸血增强的肠道微生物群提高了蚊子的抗感染能力。在这里，我们探讨了 如何改变肠道微生物群、抗感染能力，并影响肠道腔中三方 - 蚊子-微生物群相互作用。我们使用宏基因组学和 RNAseq 策略来解决这些问题。在未感染的蚊子中， 和 是主要的细菌，吸血会导致 和 的检测水平升高。对吸血中肠的平行 RNAseq 分析也显示了 转录本的存在。然而，在感染后，我们直到大约 36 小时才检测到细菌 16S rRNA。有趣的是，在第一个 36 小时内，一组选定的抗菌肽 cecropin 1-2、防御素-1 和 Gambicin 的转录表达仍然很低，此时孢子虫/早期卵囊侵入肠道。我们的结论是，在侵袭前阶段， 会与中肠微生物群竞争。这种微生物的抑制可能抵消了蚊子免疫力的影响，从而可能增强 的生存能力。检测到与蚊子相关的 序列为其作为“基于共生原转化的”蚊子控制的手段提供了新的研究方向。
