Vijay Sonam, Rawat Manmeet, Sharma Arun
Protein Biochemistry and Structural Biology Laboratory, National Institute of Malaria Research (ICMR), Sector-8, Dwarka, New Delhi 110077, India.
Protein Biochemistry and Structural Biology Laboratory, National Institute of Malaria Research (ICMR), Sector-8, Dwarka, New Delhi 110077, India ; Department of Internal Medicine, University of New Mexico School of Medicine, Albuquerque, NM 87106, USA.
Biomed Res Int. 2014;2014:686319. doi: 10.1155/2014/686319. Epub 2014 Jul 14.
Salivary gland proteins of Anopheles mosquitoes offer attractive targets to understand interactions with sporozoites, blood feeding behavior, homeostasis, and immunological evaluation of malaria vectors and parasite interactions. To date limited studies have been carried out to elucidate salivary proteins of An. stephensi salivary glands. The aim of the present study was to provide detailed analytical attributives of functional salivary gland proteins of urban malaria vector An. stephensi. A proteomic approach combining one-dimensional electrophoresis (1DE), ion trap liquid chromatography mass spectrometry (LC/MS/MS), and computational bioinformatic analysis was adopted to provide the first direct insight into identification and functional characterization of known salivary proteins and novel salivary proteins of An. stephensi. Computational studies by online servers, namely, MASCOT and OMSSA algorithms, identified a total of 36 known salivary proteins and 123 novel proteins analysed by LC/MS/MS. This first report describes a baseline proteomic catalogue of 159 salivary proteins belonging to various categories of signal transduction, regulation of blood coagulation cascade, and various immune and energy pathways of An. stephensi sialotranscriptome by mass spectrometry. Our results may serve as basis to provide a putative functional role of proteins in concept of blood feeding, biting behavior, and other aspects of vector-parasite host interactions for parasite development in anopheline mosquitoes.
按蚊的唾液腺蛋白为理解其与子孢子的相互作用、吸血行为、体内平衡以及疟疾病媒与寄生虫相互作用的免疫学评估提供了有吸引力的靶点。迄今为止，针对斯氏按蚊唾液腺的唾液蛋白开展的研究有限。本研究的目的是提供城市疟疾病媒斯氏按蚊功能性唾液腺蛋白的详细分析属性。采用了一种结合一维电泳（1DE）、离子阱液相色谱质谱联用（LC/MS/MS）和计算生物信息学分析的蛋白质组学方法，以首次直接深入了解斯氏按蚊已知唾液蛋白和新型唾液蛋白的鉴定及功能特征。通过在线服务器（即MASCOT和OMSSA算法）进行的计算研究，共鉴定出通过LC/MS/MS分析的36种已知唾液蛋白和123种新型蛋白。本首次报告描述了通过质谱分析得到的属于信号转导、凝血级联调节以及斯氏按蚊唾液转录组各种免疫和能量途径等各类别的159种唾液蛋白的基线蛋白质组目录。我们的结果可为在按蚊中寄生虫发育过程中，蛋白质在吸血、叮咬行为以及病媒-寄生虫宿主相互作用的其他方面的假定功能作用提供依据。
