Kim Sung Soo, Hur Soo Young, Kim Yoo Ri, Yoo Nam Jin, Lee Sug Hyung
Department of Internal Medicine, College of Medicine, The Catholic University of Korea, Seoul, Korea.
Tumori. 2011 May-Jun;97(3):380-5. doi: 10.1177/030089161109700321.
Aminoacyl-tRNA synthetase-interacting multifunctional proteins (AIMPs) form a protein complex with aminoacyl-tRNA synthetases. In addition to protein translation, AIMPs play a role in diverse biological processes. Earlier studies suggested that AIMPs may act as tumor suppressors. However, the expression status of the AIMP proteins in human cancer tissues is largely unknown. In this study, we analyzed the expression of AIMP members (AIMP1, AIMP2 and AIMP3) in gastric cancer (GC) and colorectal cancer (CRC) tissues. We analyzed the expression of these proteins in 100 GC and 103 CRC tissues by immunohistochemistry using a tissue microarray method. Normal gastric and colon mucosa expressed AIMP1, AIMP2 and AIMP3 in nearly all of the cases (95-100%). However, the expression of AIMP1, AIMP2 and AIMP3 was significantly decreased in the GC samples (60%, 52% and 70% of the cases, respectively) and in the CRC samples (66%, 53% and 81% of the cases, respectively) ( P <0.01). Expression of AIMP1, AIMP2 or AIMP3 was not associated with clinicopathological parameters including differentiation, depth of invasion and TNM stage. The decreased expression of AIMP1, AIMP2 and AIMP3 in the GC and CRC tissues compared to the corresponding normal tissues suggested that downregulation of these proteins may be related to inactivation of the tumor suppressor functions of AIMP proteins and might play a role in the development of GC and CRC.
氨酰-tRNA合成酶相互作用多功能蛋白（AIMPs）与氨酰-tRNA合成酶形成一种蛋白质复合物。除了蛋白质翻译外，AIMPs在多种生物学过程中发挥作用。早期研究表明，AIMPs可能作为肿瘤抑制因子。然而，AIMP蛋白在人类癌症组织中的表达状况在很大程度上尚不清楚。在本研究中，我们分析了AIMP成员（AIMP1、AIMP2和AIMP3）在胃癌（GC）和结直肠癌（CRC）组织中的表达。我们使用组织芯片方法通过免疫组织化学分析了这些蛋白质在100例GC和103例CRC组织中的表达。正常胃和结肠黏膜在几乎所有病例（95%-100%）中均表达AIMP1、AIMP2和AIMP3。然而，AIMP1、AIMP2和AIMP3的表达在GC样本（分别为60%、52%和70%的病例）和CRC样本（分别为66%、53%和81%的病例）中显著降低（P<0.01）。AIMP1、AIMP2或AIMP3的表达与包括分化、浸润深度和TNM分期在内的临床病理参数无关。与相应正常组织相比，GC和CRC组织中AIMP1、AIMP2和AIMP3表达降低表明这些蛋白质的下调可能与AIMP蛋白肿瘤抑制功能的失活有关，并且可能在GC和CRC的发生发展中起作用。
