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通过评估骨标志物研究L提取物对间充质干细胞和成骨细胞的骨再生特性：一项初步研究。

Investigating the Osteoregenerative Properties of L. Extract on Mesenchymal Stem Cells and Osteoblasts Through Evaluation of Bone Markers: A Pilot Study.

作者信息

Hanga-Fărcaș Alina, Filip Gabriela Adriana, Clichici Simona Valeria, Vicaș Laura Grațiela, Şoritău Olga, Andercou Otilia, Fritea Luminița, Mureșan Mariana Eugenia

机构信息

Doctoral School of Biomedical Sciences, University of Oradea, 1 University Street, 410087 Oradea, Romania.

Department of Physiology, Iuliu Hatieganu University of Medicine and Pharmacy, 8 Victor Babes Street, 400347 Cluj-Napoca, Romania.

出版信息

J Funct Biomater. 2025 Jul 21;16(7):268. doi: 10.3390/jfb16070268.

DOI:10.3390/jfb16070268
PMID:40710482
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12295196/
Abstract

Bone tissue regeneration is a complex process that takes place at the level of osteoblasts derived from mesenchymal cells and occurs under the action of multiple signaling pathways and through the expression of osteoregenerative markers. The leaf extract of L. (JR) is rich in polyphenols with demonstrated osteoregeneration effects. In the present study, we investigated the extract's effects on three types of cells with various stages of differentiation: adult mesenchymal stem cells (MSCs), osteoblasts at low passage (O6) and osteoblasts at advanced passage (O10). To assess the efficacy of the walnut leaf extract, in vitro treatments were performed in comparison with ellagic acid (EA) and catechin (CAT). The osteoregenerative properties of the leaf extract were evaluated in terms of cell viability, bone mineralization (by staining with alizarin red) and the expression of osteogenesis markers such as osteocalcin (OC), osteopontin (OPN), dentin matrix acidic phosphoprotein 1 (DMP1) and collagen type 1A. Another compound implicated in oxidative stress response, but also a bone homeostasis regulator, nuclear factor erythroid 2-related factor 2 (NRF2), was studied by immunocytochemistry. Together with collagen amount, alkaline phosphatase (ALP) activity and NF-kB levels were measured in cell lysates and supernatants. The obtained results demonstrate that JR treatment induced osteogenic differentiation and bone mineralization, and it showed protective effects against oxidative stress.

摘要

骨组织再生是一个复杂的过程，发生在源自间充质细胞的成骨细胞水平，在多种信号通路的作用下并通过骨再生标志物的表达而发生。光核桃叶提取物富含具有已证实的骨再生作用的多酚。在本研究中，我们研究了该提取物对三种不同分化阶段细胞的影响：成人间充质干细胞（MSCs）、低代成骨细胞（O6）和高代成骨细胞（O10）。为了评估核桃叶提取物的功效，与鞣花酸（EA）和儿茶素（CAT）相比进行了体外处理。从细胞活力、骨矿化（通过茜素红染色）以及成骨标志物如骨钙素（OC）、骨桥蛋白（OPN）、牙本质基质酸性磷酸蛋白1（DMP1）和I型胶原蛋白（collagen type 1A）的表达方面评估了叶提取物的骨再生特性。另一种与氧化应激反应有关但也是骨稳态调节剂的化合物，核因子红细胞2相关因子2（NRF2），通过免疫细胞化学进行了研究。同时在细胞裂解物和上清液中测量了胶原蛋白含量、碱性磷酸酶（ALP）活性和NF-κB水平。获得的结果表明，光核桃叶提取物处理诱导了成骨分化和骨矿化，并显示出对氧化应激的保护作用。

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