通过培养技术和酶免疫吸附测定法（IDEIA）评估沙眼衣原体感染的年龄和性别相关性。

Age and sex correlation of Chlamydia trachomatis infections evaluated by the culture technique and by an enzyme immunosorbent assay, IDEIA.

作者信息

Ostergaard L, Lundemose A G, Birkelund S, Christiansen G

机构信息

Institute of Medical Microbiology, University of Arhus, Denmark.

出版信息

Eur J Obstet Gynecol Reprod Biol. 1990 Mar;34(3):273-81. doi: 10.1016/0028-2243(90)90081-b.

DOI:10.1016/0028-2243(90)90081-b

PMID:2178993

Abstract

A total number of 1358 patients were examined for genital infections with Chlamydia trachomatis. 252 urethral smears from men and 1106 cervical smears from women were obtained. The average age of infected patients was 24.8 years (females 24.1, males 27.7). The overall prevalence was 10.6% (females 10.0%, males 13.1%). At the age below 20 years, the prevalence was 21% for both women and men, and 25% when data were restricted to patients consulting general practitioners. Above that age the overall prevalence was lower in all age intervals, and higher among males than females. All samples were tested by the tissue-culture technique, and the results were confirmed by the IDEIA (Boots-Celltech) enzyme-linked immunosorbent assay kit (EIA) for detection of C. trachomatis. The original smear was used for both culture and EIA. The EIA test was evaluated to have a sensitivity of 90.8% and a specificity of 95.4% when using a cut-off level of 0.05 mean extinction values (MEV), as described by the manufacturer.

摘要

共有1358名患者接受了沙眼衣原体生殖器感染检查。采集了252份男性尿道涂片和1106份女性宫颈涂片。感染患者的平均年龄为24.8岁（女性24.1岁，男性27.7岁）。总体患病率为10.6%（女性10.0%，男性13.1%）。在20岁以下，女性和男性的患病率均为21%，若将数据限定为咨询全科医生的患者，则患病率为25%。在该年龄以上，所有年龄段的总体患病率均较低，且男性高于女性。所有样本均采用组织培养技术进行检测，结果通过用于检测沙眼衣原体的IDEIA（Boots-Celltech）酶联免疫吸附测定试剂盒（EIA）进行确认。原始涂片用于培养和EIA检测。按照制造商的说明，当使用0.05平均消光值（MEV）的临界值时，EIA检测的灵敏度为90.8%，特异性为95.4%。