Ungvári Krisztina, Pelsoczi K István, Kormos Bernadett, Oszkó Albert, Radnai Márta, Nagy Katalin, Fazekas András, Turzó Kinga
SZTE Fogorvostudományi Kar, Fogpótlástani es Orális Biológiai Tanszék, Szeged.
Fogorv Sz. 2011 Mar;104(1):9-18.
The effects of three different decontaminating solutions in clinical use for peri-implantitis therapy on the chemical structure and surface roughness of commercially pure (CP) Ti were investigated. A further aim was to survey the response of the biological environment to these changes, by examining the attachment and proliferation of human epithelial cells after treatment of the Ti surfaces with these solutions.
CP (grade 4) machined titanium discs (CAMLOG Biotechnologies AG, Switzerland) were treated with 3% H2O2 (5 min), saturated citric acid (pH = 1; 1 min) or chlorhexidine gel (CHX, 5 min). The surface properties were followed through the use of X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). The epithelial cell attachment and proliferation was examined by means of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and bicinchoninic acid (BCA) protein-content assays.
XPS showed an intact TiO2 layer on each sample and CHX was adsorbed by the surface, as C-O and/or C=O bond formation was revealed. AFM results gave no significant changes in the roughness after treating the surfaces with the cleaning solutions. While MTT and BCA assays did not show significant differences in epithelial cell attachments, the cell proliferation was significantly increased after H2O2 treatment as compared to CHX (not shown by BCA assays).
The applied decontaminating agents do not damage the Ti surface. H2O2 can be used effectively in decontaminating the implants affected by peri-implantitis, as the human epithelial cell growth was improved, in contrast with CHX.
研究了临床用于种植体周围炎治疗的三种不同去污溶液对商业纯钛（CP Ti）化学结构和表面粗糙度的影响。另一个目的是通过检测用这些溶液处理钛表面后人类上皮细胞的附着和增殖情况，来观察生物环境对这些变化的反应。
用3%过氧化氢（5分钟）、饱和柠檬酸（pH = 1；1分钟）或洗必泰凝胶（CHX，5分钟）处理CP（4级）机械加工钛盘（瑞士CAMLOG生物技术公司）。通过X射线光电子能谱（XPS）和原子力显微镜（AFM）跟踪表面性能。通过3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴盐（MTT）和二喹啉甲酸（BCA）蛋白质含量测定法检测上皮细胞的附着和增殖情况。
XPS显示每个样品上有完整的TiO₂层，且表面吸附了CHX，因为揭示了C-O和/或C=O键的形成。AFM结果表明，用清洁溶液处理表面后粗糙度没有显著变化。虽然MTT和BCA测定在上皮细胞附着方面没有显示出显著差异，但与CHX相比，过氧化氢处理后细胞增殖显著增加（BCA测定未显示）。
所应用的去污剂不会损害钛表面。过氧化氢可有效用于对受种植体周围炎影响的种植体进行去污，因为与CHX相比，它能促进人类上皮细胞生长。
