Wiedmer David, Petersen Fernanda Cristina, Lönn-Stensrud Jessica, Tiainen Hanna
a Department of Biomaterials, Institute for Clinical Dentistry , University of Oslo , Oslo , Norway.
b Faculty of Dentistry, Department of Oral Biology , University of Oslo , Oslo , Norway.
Biofouling. 2017 Jul;33(6):451-459. doi: 10.1080/08927014.2017.1322585. Epub 2017 May 19.
The chemical decontamination of infected dental implants is essential for the successful treatment of peri-implantitis. The aim of this study was to assess the antibacterial effect of a hydrogen peroxide-titanium dioxide (HO-TiO) suspension against Staphylococcus epidermidis biofilms. Titanium (Ti) coins were inoculated with a bioluminescent S. epidermidis strain for 8 h and subsequently exposed to HO with and without TiO nanoparticles or chlorhexidine (CHX). Bacterial regrowth, bacterial load and viability after decontamination were analyzed by continuous luminescence monitoring, live/dead staining and scanning electron microscopy. Bacterial regrowth was delayed on surfaces treated with HO-TiO compared to HO. HO-based treatments resulted in a lower bacterial load compared to CHX. Few viable bacteria were found on surfaces treated with HO and HO-TiO, which contrasted with a uniform layer of dead bacteria for surfaces treated with CHX. HO-TiO suspensions could therefore be considered an alternative approach in the decontamination of dental implants.
对感染的牙种植体进行化学去污对于成功治疗种植体周围炎至关重要。本研究的目的是评估过氧化氢 - 二氧化钛（HO-TiO）悬浮液对表皮葡萄球菌生物膜的抗菌作用。将钛（Ti）硬币接种发光表皮葡萄球菌菌株8小时，随后分别暴露于含和不含TiO纳米颗粒的HO或洗必泰（CHX）中。通过连续发光监测、活/死染色和扫描电子显微镜分析去污后的细菌再生长、细菌载量和活力。与HO相比，用HO-TiO处理的表面细菌再生长延迟。与CHX相比，基于HO的处理导致细菌载量更低。在用HO和HO-TiO处理的表面上发现的存活细菌很少，这与用CHX处理的表面上均匀的死细菌层形成对比。因此，HO-TiO悬浮液可被视为牙种植体去污的一种替代方法。
