Fonterra Research Centre, Dairy Farm Road, Private Bag 11029, Palmerston North 4412, New Zealand.
J Agric Food Chem. 2011 Sep 28;59(18):9788-95. doi: 10.1021/jf202035m. Epub 2011 Aug 26.
Oligosaccharides are important components of milk with bioefficacy as prebiotics, anti-infectives, and immune system modulators and as a possible source of sialic acid for neural function. Bovine milk oligosaccharides are lower in concentration and lack the diversity of human milk oligosaccharides but could be a commercial source of milk oligosaccharides for pediatric foods. For this development, an ability to quantify the oligosaccharides is required. This study validated a hydrophilic interaction chromatography high-performance liquid chromatography-high-resolution selected reaction monitoring-mass spectrometry (HILIC HPLC-HRSRM-MS) method for measuring six different oligosaccharides in bovine milk, bovine colostrum, and infant formulas. The extraction resulted in a high recovery (90-103%) with a repeatability coefficient of variation ranging from 2 to 9% for the two dominant oligosaccharides, 3'-sialyllactose and 6'-sialyllactose, and ranging from 1 to 17% for the much lower concentration oligosaccharides, 6'-sialyllactosamine, disialyllactose, and N-acetylgalactosaminyllactose. The sixth oligosaccharide, 3'-sialyllactosamine, was not detected in any of the samples.
低聚糖是牛奶的重要组成部分，具有作为益生元、抗感染和免疫系统调节剂的生物功效，并且可能是神经功能唾液酸的来源。牛乳低聚糖的浓度较低，且缺乏人乳低聚糖的多样性，但它可能是儿科食品用乳低聚糖的商业来源。为此发展，需要能够定量测定低聚糖的能力。本研究验证了一种亲水作用色谱-高效液相色谱-高分辨选择反应监测-质谱（HILIC HPLC-HRSRM-MS）法，用于测量牛乳、牛初乳和婴儿配方奶粉中的六种不同的低聚糖。该提取方法具有高回收率（90-103%），两种主要低聚糖 3'-唾液酸乳糖和 6'-唾液酸乳糖的重复性变异系数范围为 2-9%，而浓度较低的低聚糖 6'-唾液酸乳糖胺、二唾液酸乳糖和 N-乙酰半乳糖胺乳糖的变异系数范围为 1-17%。在任何样品中均未检测到第六种低聚糖 3'-唾液酸乳糖胺。
