Moselhy W A, Helmy N A, Abdel-Halim B R, Nabil T M, Abdel-Hamid M I
Department of Forensic Medicine and Toxicology, Beni-Suef University, Beni-Suef, Egypt.
Reprod Domest Anim. 2012 Apr;47(2):335-43. doi: 10.1111/j.1439-0531.2011.01878.x. Epub 2011 Jul 26.
The aim of the current study was to evaluate reproductive disorders concomitant with aluminium chloride (AlCl(3) ) toxicity in Albino male rats. Attention was also directed to study the protective influence of ginger against this toxicity. Forty-five mature Albino Wistar male rats were equally divided into three groups; the first group was served as control group while those of the second group (AlCl(3) ) were daily treated with 34 mg/kg bw. AlCl(3) orally. The third group (AlCl(3) + ginger) was treated daily with AlCl(3) as in group 2 in combination with ginger (40 mg/kg bw), which started 2 weeks prior to AlCl(3) . Five animals from each group were sacrificed on days 30, 45 and 60 of treatment. AlCl(3) administration significantly decreased serum testosterone levels, increased testicular homogenate malondialdehyde and deteriorated semen picture with increased testicular DNA fragmentation. Histopathological examination revealed degenerative changes of the seminiferous tubules with focal areas of necrosed spermatogenic cells, marked degeneration and desquamation of the lining epithelial cells of epididymis as well as multiple calcified material in prostate gland following 60 days of aluminium treatment. Ginger treatment started to improve significantly all studied parameters after 60 days as compared with AlCl(3) -treated group. In the current study, it was concluded that AlCl(3) had a destructive effect on all the studied reproductive parameters. Treatment with ginger has an ameliorating effect against AlCl(3) toxicity after 60 days post-treatment.
本研究的目的是评估白化雄性大鼠中与氯化铝（AlCl₃）毒性相关的生殖障碍。同时也关注研究生姜对这种毒性的保护作用。45只成熟的白化Wistar雄性大鼠被平均分为三组；第一组作为对照组，而第二组（AlCl₃组）每天口服34 mg/kg体重的AlCl₃。第三组（AlCl₃ + 生姜组）每天按照第二组的方式给予AlCl₃，并联合生姜（40 mg/kg体重），生姜在AlCl₃给药前2周开始使用。在治疗的第30天、45天和60天，每组处死5只动物。给予AlCl₃显著降低了血清睾酮水平，增加了睾丸匀浆丙二醛含量，使精液情况恶化，并增加了睾丸DNA片段化。组织病理学检查显示，在铝处理60天后，生精小管出现退行性变化，伴有局灶性坏死的生精细胞区域，附睾衬里上皮细胞明显变性和脱落，前列腺出现多处钙化物质。与AlCl₃处理组相比，生姜处理在60天后开始显著改善所有研究参数。在本研究中，得出的结论是AlCl₃对所有研究的生殖参数都有破坏作用。生姜治疗在治疗后60天对AlCl₃毒性有改善作用。
