Bordbar Hossein, Esmaeilpour Tahereh, Dehghani Farzaneh, Panjehshahin Mohammad Reza
Department of Anatomy, Shiraz University of Medical Sciences, Shiraz, Iran. ; Histomorphometry, Stereology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Department of Anatomy, Shiraz University of Medical Sciences, Shiraz, Iran.
Iran J Reprod Med. 2013 Jun;11(6):467-72.
In traditional medicine zingiber officinale used to regulate female menstural cycle and treat male infertility. Recent studies have suggested the possible role of ginger extract in improving the testicular damage of busulfan.
The aim of this study was to evaluate the effects of zingiber officinale on the sperm parameters, testosterone level and the volume of the testes and seminiferous tubules by stereological methods.
Fifty rats were divided into four groups. All the rats were given a single intraperitoneally injection of 5mg/kg busulfan solution. The first group was kept as busulfan control, while the other groups were orally administrated ginger extract in graded doses of 50, 100 and 150mg/kg b.wt, for 48 consecutive days. At the end, all animals were anesthetized and their testes and vas deference were removed, fixed, embedded, and stained. The volume of testes and seminiferous tubules were estimated by cavalieri methods.
The result showed, that zingiber officinale increased the volumes of seminiferous tubule in 100mg/kg treated group compared to control group. Sperm count (706×10(5) and 682×10(5)) and the level of testosterone (50.90 ng/mL and 54.10 ng/mL) enhanced in 100 mg/kg and 150 mg/kg treated groups compared to control group (p=0.00).
It seems that zingiber officinale stimulate male reproductive system in induce busulfan infertility.
在传统医学中，生姜用于调节女性月经周期并治疗男性不育症。最近的研究表明，生姜提取物可能对改善白消安所致的睾丸损伤有作用。
本研究旨在通过体视学方法评估生姜对精子参数、睾酮水平以及睾丸和生精小管体积的影响。
将50只大鼠分为四组。所有大鼠均腹腔注射一次5mg/kg的白消安溶液。第一组作为白消安对照组，其他组分别以50、100和150mg/kg体重的剂量连续48天口服给予生姜提取物。最后，对所有动物进行麻醉，取出其睾丸和输精管，固定、包埋并染色。采用卡瓦列里方法估计睾丸和生精小管的体积。
结果显示，与对照组相比，100mg/kg治疗组的生姜增加了生精小管的体积。与对照组相比，100mg/kg和150mg/kg治疗组的精子计数（分别为706×10⁵和682×10⁵）和睾酮水平（分别为50.90ng/mL和54.10ng/mL）有所提高（p = 0.00）。
生姜似乎能刺激男性生殖系统，改善白消安所致的不育症。
