Institut für Pharmazeutische Technologie, Technische Universität Braunschweig, Braunschweig, Germany.
Eur J Pharm Biopharm. 2011 Aug;78(3):432-40. doi: 10.1016/j.ejpb.2011.01.022. Epub 2011 Feb 15.
The limited source of human nail plate for studying drug permeation inspired us to develop a nail plate model made of human hair keratin. The manufacturing process consisted of keratin extraction, dialysis, molding, solvent evaporation, and curing, producing a water-resistant film. The permeability of the film was examined using three markers: sodium fluorescein, rhodamine B, and fluorescein isothiocyanate-dextran as water-soluble, lipid-soluble, and large molecule models, respectively. Bovine hoof was used for comparison. First investigation showed that keratin films (thickness 120 μm) resembled hooves (thickness 100 μm) except that these films were more permeable to rhodamine B compared with hooves (1.8-fold, p<0.01). Subsequent investigations using ungual penetration enhancers (urea, thioglycolic acid, and papain) showed that keratin films were generally more susceptible than hooves. This study revealed that the produced keratin film offers a possibility as a human nail plate substitute. However, inclusion of the penetration enhancer must be carefully interpreted.
人类指甲板的有限来源限制了我们对药物渗透的研究，这启发我们开发了一种用人发角蛋白制成的指甲板模型。该模型的制造过程包括角蛋白提取、透析、成型、溶剂蒸发和固化，最终生成一种耐水的薄膜。我们使用三种标记物（分别为水溶性的荧光素钠、脂溶性的罗丹明 B 和大分子模型异硫氰酸荧光素标记葡聚糖）来检测薄膜的渗透性。同时还使用牛蹄进行了对比。初步研究表明，角蛋白薄膜（厚度为 120μm）与牛蹄（厚度为 100μm）相似，只是角蛋白薄膜对罗丹明 B 的渗透性比牛蹄高 1.8 倍（p<0.01）。随后使用指甲穿透增强剂（尿素、巯基乙酸和木瓜蛋白酶）进行的研究表明，角蛋白薄膜通常比牛蹄更敏感。本研究表明，所制备的角蛋白薄膜为替代人类指甲板提供了一种可能性。但是，必须谨慎解释渗透增强剂的应用。
