Food Protectants & Infestation Control Department, Central Food Technological Research Institute, Mysore, 570020, India.
Environ Toxicol Pharmacol. 2008 Sep;26(2):241-6. doi: 10.1016/j.etap.2008.05.006. Epub 2008 Jun 8.
Our earlier studies have shown that extracts derived from potato peel (PPE) are rich in polyphenols and possess strong antioxidant activity both in vitro and in vivo. The objective of the present study was to investigate its potential to offer protection against acute liver injury in rats. Rats pretreated with PPE (oral, 100mg/kgb.w./day for 7 days) were administered a single oral dose carbon tetrachloride (CCl(4), 3ml/kg b.w., 1:1 in groundnut oil) and sacrificed 8h of post-treatment. Hepatic damage was assessed by employing biochemical parameters (transaminase enzyme levels in plasma and liver [AST-aspartate transaminase; ALT-alanine transaminase, LDH-lactate dehydrogenase]). Further, markers of hepatic oxidative damage were measured in terms of malondialdehyde (MDA), enzymic antioxidants (CAT, SOT, GST, GPX) and GSH (reduced glutathione) levels. In addition, the CCl(4)-induced pathological changes in liver were evaluated by histopathological studies. Our results demonstrated that pretreatment of rats with PPE significantly prevented the increased activities of AST and ALT in serum, prevented the elevation of hepatic MDA formation as well as protected the liver from GSH depletion. PPE pretreatment also restored CCl(4)-induced altered antioxidant enzyme activities to control levels. The protective effect of PPE was further evident through the decreased histological alterations in liver. Our findings provide evidences to demonstrate that PPE pretreatment significantly offsets CCl(4)-induced liver injury in rats, which may be attributable to its strong antioxidant propensity.
我们之前的研究表明，从土豆皮中提取的物质（PPE）富含多酚，具有很强的体外和体内抗氧化活性。本研究的目的是研究其对大鼠急性肝损伤的保护作用。大鼠用 PPE 预处理（口服，每天 100mg/kgb.w.，共 7 天），然后给予单次口服四氯化碳（CCl(4)，3ml/kgb.w.，用花生油 1:1 稀释），并在治疗后 8 小时处死。通过生化参数（血浆和肝脏中转氨酶酶水平[AST-天冬氨酸转氨酶；ALT-丙氨酸转氨酶，LDH-乳酸脱氢酶]）评估肝损伤。此外，还通过丙二醛（MDA）、酶抗氧化剂（CAT、SOT、GST、GPX）和 GSH（还原型谷胱甘肽）水平来衡量肝氧化损伤的标志物。此外，还通过组织病理学研究评估 CCl(4)诱导的肝损伤的病理变化。我们的结果表明，PPE 预处理可显著降低血清中 AST 和 ALT 的活性，防止肝 MDA 形成升高，并防止 GSH 耗竭。PPE 预处理还可使 CCl(4)诱导的抗氧化酶活性恢复到对照水平。PPE 的保护作用还通过降低肝组织学改变得到进一步证实。我们的研究结果表明，PPE 预处理可显著减轻 CCl(4)诱导的大鼠肝损伤，这可能与其较强的抗氧化倾向有关。
