Alhassan Hassan H, Al-Keridis Lamya Ahmed, Ayub Huma, Alenazy Fawaz O, Alruwaili Yasir, Khan Muhammad Rashid, Fatima Mehreen, Patel Mitesh, Alshammari Nawaf, Adnan Mohd, Sahreen Sumaira
Department of Clinical Laboratory Sciences, College of Applied Medical Sciences-Sakaka, Jouf University, Saudi Arabia.
Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh, 11671, Saudi Arabia.
Heliyon. 2023 Aug 19;9(8):e19324. doi: 10.1016/j.heliyon.2023.e19324. eCollection 2023 Aug.
Fruit of Stapf ex Haines ( is a feed additive and is commonly used against cardiac dysfunction, fever, asthma, diarrhea, gastrointestinal ailments, and skin diseases. In this study, we aimed to evaluate the metabolic profile and antioxidant potential of fruit against carbon tetrachloride (CCl)-induced cardiotoxicity and testicular toxicity in rats. Gas Chromatoghraphy-Mass Spectrometry (GC-MS) analysis of fruit for the identification of potential metabolic profile, followed by methanolic extract of and its derived fractions including n-hexane, ethyl acetate, chloroform, butanol, and aqueous were used to assess the antioxidant potential of fruits. Ten groups of rats received different treatments and got evaluated for cardiac and testicular antioxidant enzymes, histological architecture, and serum hormonal levels. GC-MS analysis of methanolic extract of fruit showed the presence of some bioactive metabolites like cyclodecane, diethyl 2,6-pyridine dicarboxylate, tetrahydro-geraniol, S-[2-[N, N-Dimethylamino]ethyl]morpoline, 2,3-Methylenedioxyphenol, alpha-d-Glucopyranoside, 5,10-Diethoxy-2,3,7,8-tetrahydro-1H, 6H-dipyrrolo [1,2-a; 1',2'-d] pyrazine and 1,3-Benzothiazol-2(3H)-one,3-(3,3-dimethyl-1-oxobutyl) that corresponds the medicinal properties of fruit. Prepared fractions of fruits mitigated the toxicity induced by CCl in the heart and testicular tissues of rats. Oxidative stress was caused by the inhibition of activities of glutathione and other antioxidant enzymes of the body, while on the other hand elevating the levels of nitrite and hydrogen peroxide. Treatment with fruit extract normalized the levels of enzymes, reproductive hormones, and free radicals thus restoring the histopathological and enzymatic biomarkers towards the normal group. The study supports the indigenous use of fruits as an alternative medicine against cardiac dysfunction by providing scientific evidence of protection against CCl-induced injuries, and it also concludes the antioxidant defensive role in testicular tissues.
海恩斯 Stapf 果实（是一种饲料添加剂，常用于治疗心脏功能障碍、发烧、哮喘、腹泻、胃肠道疾病和皮肤病。在本研究中，我们旨在评估海恩斯 Stapf 果实对四氯化碳（CCl）诱导的大鼠心脏毒性和睾丸毒性的代谢谱和抗氧化潜力。采用气相色谱 - 质谱联用（GC-MS）分析法对海恩斯 Stapf 果实进行分析以确定潜在的代谢谱，随后使用海恩斯 Stapf 果实的甲醇提取物及其衍生馏分（包括正己烷、乙酸乙酯、氯仿、丁醇和水相）来评估果实的抗氧化潜力。将十组大鼠进行不同处理，并对其心脏和睾丸抗氧化酶、组织学结构以及血清激素水平进行评估。海恩斯 Stapf 果实甲醇提取物的 GC-MS 分析表明存在一些生物活性代谢物，如环癸烷、2,6 - 吡啶二甲酸二乙酯、四氢香叶醇、S - [2 - [N, N - 二甲基氨基]乙基]吗啉、2,3 - 亚甲二氧基苯酚、α - d - 吡喃葡萄糖苷、5,10 - 二乙氧基 - 2,3,7,8 - 四氢 - 1H, 6H - 二吡咯并[1,2 - a; 1',2'- d]吡嗪和 1,3 - 苯并噻唑 - 2(3H)- 酮，3 - (3,3 - 二甲基 - 1 - 氧代丁基），这些与海恩斯 Stapf 果实的药用特性相符。制备的海恩斯 Stapf 果实馏分减轻了 CCl 对大鼠心脏和睾丸组织诱导的毒性。氧化应激是由体内谷胱甘肽和其他抗氧化酶活性的抑制引起的，而另一方面会提高亚硝酸盐和过氧化氢的水平。用海恩斯 Stapf 果实提取物处理可使酶、生殖激素和自由基水平恢复正常，从而使组织病理学和酶生物标志物恢复到正常组水平。该研究通过提供针对 CCl 诱导损伤的保护的科学证据，支持了将海恩斯 Stapf 果实作为治疗心脏功能障碍的替代药物的本土用途，并且还得出了其在睾丸组织中的抗氧化防御作用。
