Centre for Phytochemistry and Pharmacology, Southern Cross University, Lismore, P.O. Box 157, Lismore, NSW 2480, Australia.
Evid Based Complement Alternat Med. 2011;2011:598521. doi: 10.1093/ecam/neq012. Epub 2011 Jan 9.
Chlorophytum borivilianum Santapau & Fernandes (Liliaceae) is an ayurvedic Rasayana herb with immunostimulating properties. The polysaccharide fraction (CBP) derived from hot water extraction of C. borivilianum (CB), comprising of ∼31% inulin-type fructans and ∼25% acetylated mannans (of hot water-soluble extract), was evaluated for its effect on natural killer (NK) cell activity (in vitro). Human peripheral blood mononuclear cells (PBMCs), isolated from whole blood on a Ficoll-Hypaque density gradient, were tested in the presence or absence of varying concentrations of each C. borivilianum fraction for modulation of NK cell cytotoxic activity toward K562 cells. Preliminary cytotoxicity evaluation against P388 cells was performed to establish non-cytotoxic concentrations of the different fractions. Testing showed the observed significant stimulation of NK cell activity to be due to the CBP of C. borivilianum. Furthermore, in vivo evaluation carried out on Wistar strain albino rats for humoral response to sheep red blood cells (SRBCs) and immunoglobulin-level determination using enzyme-linked immunosorbent assay (ELISA), exhibited an effectiveness of C. borivilianum aqueous extract in improving immune function. Present results provide useful information for understanding the role of CBP in modulating immune function.
尼泊尔天门冬（百合科）是一种具有免疫刺激特性的阿育吠陀罗莎亚那草药。从尼泊尔天门冬热水提取物中获得的多糖部分（CBP），由约 31%的菊粉型果聚糖和约 25%的乙酰化甘露聚糖（热水溶性提取物）组成，评估其对自然杀伤（NK）细胞活性（体外）的影响。从 Ficoll-Hypaque 密度梯度的全血中分离出人外周血单核细胞（PBMC），在存在或不存在不同浓度的每种尼泊尔天门冬部分的情况下，测试其对 K562 细胞的 NK 细胞细胞毒性活性的调节作用。对 P388 细胞进行初步细胞毒性评估，以确定不同部分的非细胞毒性浓度。测试表明，观察到的 NK 细胞活性的显著刺激是由于尼泊尔天门冬的 CBP。此外，对 Wistar 品系白化大鼠进行体液对绵羊红细胞（SRBC）的反应和免疫球蛋白水平的酶联免疫吸附测定（ELISA）评估，显示尼泊尔天门冬水提取物在改善免疫功能方面的有效性。目前的结果为了解 CBP 在调节免疫功能中的作用提供了有用的信息。
