Department of Agronomy, Purdue University, West Lafayette, IN 47907, USA.
Theor Appl Genet. 2011 Oct;123(6):985-97. doi: 10.1007/s00122-011-1641-5. Epub 2011 Jul 27.
The partially dominant, autoactive maize disease resistance gene Rp1-D21 causes hypersensitive response (HR) lesions to form spontaneously on leaves and stems in the absence of pathogen recognition. The maize nested association mapping (NAM) population consists of 25 200-line subpopulations each derived from a cross between the maize line B73 and one of 25 diverse inbred lines. By crossing a line carrying the Rp1-D21 gene with lines from three of these subpopulations and assessing the F(1) progeny, we were able to map several novel loci that modify the maize HR, using both single-population quantitative trait locus (QTL) and joint analysis of all three populations. Joint analysis detected QTL in greater number and with greater confidence and precision than did single population analysis. In particular, QTL were detected in bins 1.02, 4.04, 9.03, and 10.03. We have previously termed this technique, in which a mutant phenotype is used as a "reporter" for a trait of interest, Mutant-Assisted Gene Identification and Characterization (MAGIC).
部分显性、自动激活的玉米疾病抗性基因 Rp1-D21 导致在没有病原体识别的情况下，叶片和茎部会自发形成过敏反应 (HR) 损伤。玉米嵌套关联图谱 (NAM) 群体由 25200 个亚群体组成，每个亚群体均源自玉米系 B73 与 25 个不同自交系的杂交。通过将携带 Rp1-D21 基因的系与这三个亚群体中的三个系杂交，并评估 F1 后代，我们能够利用单亚群体数量性状位点 (QTL) 和对所有三个群体的联合分析，来对几种修饰玉米 HR 的新基因座进行定位。联合分析比单群体分析检测到了更多、更有信心和更精确的 QTL。特别是，在 bin1.02、4.04、9.03 和 10.03 中检测到了 QTL。我们之前将这种技术称为突变体辅助基因鉴定和特征描述 (MAGIC)，即利用突变体表型作为感兴趣性状的“报告器”。
