Department of Mechanical and Aerospace Engineering, University of California, Irvine, USA.
Electrophoresis. 2011 Sep;32(17):2385-92. doi: 10.1002/elps.201100059. Epub 2011 Jul 27.
Carbon-electrode dielectrophoresis (carbon-DEP) is demonstrated here as an alternative to more traditional DEP techniques. Carbon-DEP combines advantages of metal-electrode and insulator-based DEP by using low-cost fabrication techniques and low voltages for particle manipulation. The use of 3-D electrodes is proved to yield significant advantages over the use of traditional planar electrodes. This paper details the fabrication of dense arrays of tall high aspect ratio carbon electrodes on a transparent fused-silica substrate. The shrinkage of the SU-8 structures during carbonization is characterized and a design tool for future devices is provided. Applications of carbon electrodes in DEP are then detailed and include particle positioning, high-throughput filtering and cell focusing using positive-DEP. Manipulated cells include Saccharomyces cerevisiae and Drosophila melanogaster. The advantages and disadvantages of carbon-DEP are discussed at the end of this work.
碳电极介电泳（carbon-DEP）在此被证明是一种替代更传统的介电泳技术的方法。碳-DEP 通过使用低成本制造技术和低电压来进行粒子操作，结合了金属电极和基于绝缘体的介电泳的优点。使用 3D 电极被证明比使用传统平面电极具有显著的优势。本文详细介绍了在透明熔融石英基底上制造密集排列的高纵横比碳电极的方法。对 SU-8 结构在碳化过程中的收缩进行了表征，并为未来的器件提供了设计工具。然后详细介绍了碳电极在介电泳中的应用，包括使用正介电泳进行粒子定位、高通量过滤和细胞聚焦。被操纵的细胞包括酿酒酵母和黑腹果蝇。本文最后讨论了碳-DEP 的优缺点。
