Martinez-Duarte Rodrigo
Department of Mechanical Engineering, Multiscale Manufacturing Laboratory, Clemson University, 204 Fluor Daniel, Clemson, SC 29672, USA.
IET Nanobiotechnol. 2017 Mar;11(2):127-133. doi: 10.1049/iet-nbt.2016.0154.
The focus of this review is to assess the current status of three-dimensional (3D) carbon-electrode dielectrophoresis (carbonDEP) and identify the challenges currently preventing it from its use in high-throughput applications such as sample preparation for diagnostics. The use of 3D electrodes over more traditional planar ones is emphasised here as a way to increase the throughput of DEP devices. Glass-like carbon electrodes are derived through the carbonisation of photoresist structures made using photolithography. These biocompatible carbon electrodes are not ideal electrical conductors but are more electrochemically stable than noble metals such as gold and platinum. They are also significantly less expensive than common electrode materials, both in terms of material cost and fabrication process. CarbonDEP has been demonstrated for the manipulation of microorganisms and biomolecules. This review is divided in three main sections: (i) carbonDEP fabrication process; (ii) applications using 3D carbonDEP; and (iii) challenges and perspectives on the use of carbonDEP for high-throughput applications.
本综述的重点是评估三维（3D）碳电极介电电泳（carbonDEP）的现状，并确定目前阻碍其用于高通量应用（如诊断样品制备）的挑战。本文强调使用3D电极而非更传统的平面电极，以此作为提高DEP设备通量的一种方式。类玻璃碳电极是通过对使用光刻技术制作的光刻胶结构进行碳化而得到的。这些生物相容性碳电极并非理想的电导体，但比金和铂等贵金属具有更高的电化学稳定性。在材料成本和制造工艺方面，它们也比普通电极材料便宜得多。carbonDEP已被证明可用于操纵微生物和生物分子。本综述分为三个主要部分：（i）carbonDEP制造工艺；（ii）使用3D carbonDEP的应用；（iii）将carbonDEP用于高通量应用的挑战与前景。
